抗生素的发现是人类对抗病原菌感染的重大里程碑，但是传统抗生素是通过阻断细菌中共有的信号通路或新陈代谢途径来起作用的，往往无法区分杀菌对象，长期使用会导致菌群失调及耐药性的产生，因此我们需要找出一种新型抗菌药物能特异针对病原菌或耐药菌。CRISPR-Cas9系统来源于细菌的获得性免疫，能够利用RNA与Cas相关蛋白识别特异DNA序列并对其进行切割。与传统基因编辑工具如锌指核酸内切酶(ZFNs)和类转录激活因子(TALENs)相比具有简单，高效的优点。因此作为一种新型的基因编辑工具被广泛的应用于各个领域对基因进行改造如敲除，插入和点突变。另一方面，研究人员利用其对基因的特异性识别，从基因水平上识别...The discovery of antibiotic is the great milestone for fighting against the bacterial infection. However, the traditional antibiotic works by blocking conserved bacterial signaling or metabolic pathways, therefore it is unable to distinguish between the pathogen needed to be killed and the bacteria being good for us, Long-termed usage will lead to dysbacteriosis and drug-resistance. We need to fin...学位：医学硕士院系专业：公共卫生学院_转化医学学号：3262014115059
