目的茅苍术水煎液和它的含药血清对H9c2该细胞的保护效果的研究。方法采用双氧水制造H9c2大鼠心肌细胞的氧化损伤模型,以Vc为阳性对照,通过对受损心肌细胞形态学观测、SOD活力检测及细胞相对凋亡情况的检测,测定茅苍术水煎液和它的含药血清对H9c2细胞的保护效果。结果不同浓度的茅苍术水煎液均使细胞生长状态得以改善,外部形态明显好转。高剂量组细胞生长较好,中剂量组次之,低剂量组较差;茅苍术水煎液组LDH释放量和MDA含量明显低于Vc对照组,SOD活力明显高于损伤组;细胞的相对存活率随着茅苍术水提液的浓度升高而明显增大,分别为72.37%、66.19%、55.44%,低剂量组的茅苍术水提液对H2O2损伤的H9c2心肌细胞的保护作用不明显。结论茅苍术水提液的保护作用机制与增强细胞抗氧化能力、减少自由基和脂质过氧化物导致的细胞膜损伤有关。Objective To investigate the protective activity of Atractylodes lancea decoction and its medicated serum on H9c2 cell.Methods The H2O2-based oxidative injury model was established by cultivating rat cardiomyocytes of H9c2 in vitro,and the antioxidant activity was evaluated by cell morphology,apoptosis and the effect on SOD.Results The growth state of the cells could be improved by the decoction of the different concentrations of Atractylodes lancea,and the external form was significantly improved.The high dose group cells grew well,and the low dose group was poor.The release amount of LDH and MDA of Atractylodes lancea group were significantly lower than those of the Vc control group,SOD activity was significantly higher than that in the injury group,the relative survival rate of cells with the concentration of aqueous extract of Atractylodes lancea obviously increased,it was 72.37%,66.19% and 55.44%,respectively.The protective effect of the aqueous extract of the low dose group on the H2O2 damage of H9c2 myocardial cells was not significant.Conclusion The mechanism of protective effect of water extract of Atractylodes lancea liquid is related to enhancing cellular antioxidant capacity and cell membrane damage caused by reducing free radicals and lipid peroxide
