水作为所有细胞和组织的重要组成部分,其pH和诸多生理活动密切相关.荧光分析具有可视化、高分辨率等特点,在生命体系的pH分析中得到广泛应用.近年来; , pH触发荧光成像技术在生物医学领域逐渐得到应用.本文简要介绍了一类酸度触发荧光、含分子内螺环的罗丹明衍生物的设计与功能化,及其生物成像应用.pH is critical for diverse physiological activities. Disruption of pH; homeostasis is pertinent to myriad pathological events. Particularly,; the organelle-specific pH within mammalian cells is elaborately; controlled and yet succumbs to pathological stress. Lysosomes are the; major intracellular acidic compartments, and the lysosomal acidity is; critical for diverse biological events. Aberrant lysosomal pH is; manifested a broad spectrum of diseases ranging from inflammation to; cancer metastasis, and to lysosomal storage diseases. As such, defining; pH changes in specific pathological settings would be of use for; biomedical investigations. Rhodamine-lactams, a group of nonfluorescent; rhodamine derivatives featuring intramolecular spirolactam, are poised; to proton triggered opening of the lactams to give fluorescent; rhodamines, and has been widely used for lysosomal imaging in cell; models. Herein we review the use of structurally modified; rhodamine-lactams as acidity-reporting entities for bioimaging of; distinct biological events, including host-pathogen interaction, cell; death, autophagy, and mitochondrial depolarization in cells, and; inflammation and tumors in mice models.国家自然科学基金; 福建省自然科学基金; 中央高校基本科研业务费专项资
