以典型水华藻铜绿微囊藻、针杆藻和四尾栅藻为研究对象,利用大气压强电离放电高效生成的羟基自由基(·OH)对3种藻进行杀灭.采用荧光染色、流式细胞仪; 和光合活性等生物学方法,确定·OH杀灭的阈值浓度和时间,并观察细胞形态变化.结果表明,当混合藻中铜绿微囊藻、针杆藻和四尾栅藻的初始藻密度分别为1; 9.5*10~4、21.8*10~4和4.90*10~4cells/mL时,; ·OH杀灭的阈值浓度为1.07mg/L,致死时间为4.5s;形态观察结果表明,处理后各种藻的形态是完整的,无内溶质溢出.因此,采用·OH可实现高; 效快速杀灭水华藻,有效保障饮用水安全.Algae blooming in water sources breaks out increasingly and seriously; threatened the water supply safety. Bench scale tests were conducted to; study the effects of ·OH generated from strong ionization discharge and; high pressure water jet cavitation on cell density, cell integrity and; photosynthetic capacity of 3kinds of typical freshwater algae. Algae; species including Microcystis aeruginosa, Synedra sp., and Scenedesmus; quadricuauda were respectively prepared at concentrations of 19.5*10~4,; 21.8*10~4 and 4.90*10~4 cells/mL, and the cell integrity was assessed by; flow cytometry. Results suggested that the ·OH lethal threshold of the; algae was 1.07mg/L within the exposure time of 4.5s. The cell; morphological observation results showed that all the cells were; integral and no cytoplasm composition spilled. Hence, large-scale; production of ·OH is a novel method to inactive typical algae species; efficiently and to protect drinking water safety simultaneously.国家科技支撑计划资助项目; 国家重大科研仪器研制项
