作为常见的肺螺亚纲生物,日本菊花螺Siphonaria japonica(Donovan,1824)会将卵带产于潮间带岩石上,因此会经常遭受降水的影响。为阐明降水对日本菊花螺胚胎发育和幼体孵化的影响,本研究通过实验室模拟和野外原位取样相结合的方式分析了降水对卵带内胚胎发育状态、幼体累积孵化数、单位时间幼体孵化最高值和最高值出现时间的影响。结果表明:实验室模拟降水会显著影响卵带胚胎发育和幼体孵化(P0.05),但会显著加快幼体孵化速率(P<0.05)。国家自然科学基金资助项目(41476115)；福建省杰出青年基金项目滚动项目(2017J07003)~~

丁孟文

王伟

董云伟

English

Xiamen University Institutional Repository

  Marine Sciences / Vol. 41, No. 11 / 2017 67 降水对日本菊花螺胚胎发育和幼体孵化的影响 王  伟1, 丁孟文1, 董云伟1, 2 (1. ???? ??????? ???????????????, ?? ?? 361102; 2. ?????????????????, ?? ?? 361102) 摘要: 作为常见的肺螺亚纲生物, 日本菊花螺 Siphonaria japonica(Donovan, 1824)会将卵带产于潮间带岩石上, 因此会经常遭受降水的影响。为阐明降水对日本菊花螺胚胎发育和幼体孵化的影响, 本研究通过实验室模拟和野外原位取样相结合的方式分析了降水对卵带内胚胎发育状态、幼体累积孵化数、单位时间幼体孵化最高值和最高值出现时间的影响。结果表明: 实验室模拟降水会显著影响卵带胚胎发育和幼体孵化(P<0.05); 自然降水并不会影响幼体累积孵化数(P>0.05), 但会显著加快幼体孵化速率(P<0.05)。 关键词: 日本菊花螺 Siphonaria japonica (Donovan, 1824); 卵带; 降水; 胚胎发育; 幼体孵化 中图分类号: P735    文献标识码: A      文章编号: 1000-3096(2017)11-0067-08 DOI: 10.11759/hykx20170713002 ????? Siphonaria japonica(Donovan, 1824)????????(Mollusca)????(Gastropoda)?????(Pulmonata), ??????????????????????????[1-4]????????????????, ??????????????????????????, ????????????????????????[5]?????, ???????????????, ?????????????????????????, ?????????[6]? ?????????????????????????[7], ??????????, ???????????????????????????????????????????????????????, ??????(???)??????????????????? 5???[8]??? 4~20 d?????? , ?????????? , ??????, ????????????[1]?????????????: ?? , ??????????????????????[9]; ??, ??????????????????? [9], ??????????????????????????????[10]???, ??????????????????????, ??????????????????????????????, ?????????????????[10]? ???????????????????????[11-13]?????????????, ????????????????????????????????????????, ?????????[10, 14-18]???????????????????????????????????????, ?????????????????????????????????, ?????????????????????????????????????????????, ????????????????????????????, ????????????????????????????????? 1  材料与方法 1.1  实验材料 ????????????????????               ????: 2017-07-03; ????: 2017-08-30 ????: ????????????(41476115); ???????????????(2017J07003) [Foundation: National Natural Science Foundation of China, No. 41476115; Nature Science funds for distinguished Young Scholars of Fujian Province, China, No. 2017J07003] ????: ??(1988-), ? , ????? , ?????, ????????????????? , ??: 15959229317, E-mail: snowma-nathome@126.com; ???, ????, ????????????????, ??: 18659211278, E-mail: dongyw@xmu.edu.cn  68 ????  / 2017?  / ? 41?  / ? 11? ????????, ??? 2015? 3? 17????????(118°14′E, 24°42′N), ?? 15.89 mm±1.66 mm, ?? 11.37 mm±1.49 mm, ?? 4.77 mm±0.56 mm, ?? 493.49 mg±158.49 mg(???±???, n=30)??????????: ????????? 24 °C; ??? 28; ????? 12 h: 12 h; ?????? 2 h, ???????????????? 1.2 实验方法 1.2.1  ??????????????? ??????????? 2015 ? 3 ? 22 ??? 32 ?????????, ???????, ??????????????(???)???????? 2?, ?????????(???????)? 16??? , ??????????????? , ??????(?? 28)???(?? 0)?? 1 h???????, ???????????????(?? 6 cm)?, ?? 20 mL?? 0.22 μL??????(?? 28), ???????????(??? 24℃, ?????12 h︰12 h, ?? 1 d? 1???)????????? 12?36?72? 96 h?(???????? 4?????, ????????????????????????????????), ?????????, ????? 4 ????????????????????????????????? 5 ???????, ???????? 20 ???????????? 100 ????????, ?????????????????????????????Ocaña?Emson???[8]: ???????????????; ?????????????????????; 厴???????? ??????????????? 1.2.2  ?????????????? ??????????? 3 ? 25 ??? 30 ????????????????????????(? 15 ???), ??????(?? 28)???(?? 0)?? 1 h??????, ????????????????? , ???????????? , ?? 20 mL?????????? 1.2.1???????, ?? 1 d ?? 1 ?, ??????, ??????????????????: ?? 1 mL????????????????????????, ?????????(20 mL)?? 3? 1 mL???, ??????, ??? 20 mL ???, ????????(2 d)?????????????? 1.2.3  ?????????????? ? 2015? 4? 20? 16: 00~23: 00, ?????7 h??, ????? 10 mm, ??????(????? GB/T 28592-2012, (5~14.9) mm/12 h ? (10~ 24.9) mm/24 h), ????????? 350 cm (????????, ?????????????? 300~ 400 cm???????), ???????????????????????????????????????, ?????????????????4? 21????????????? 70?, ???????????, ?????????????????????????? 10 ?, ?????, ??? A1?A2?A3?A4?????????????, ?????????????, ?? 20 mL?????????? 1.2.1 ???????, ??1 d?? 1?, ????????????????(????? 1.2.2???)? ?? 4? 21??4? 28?????, ??? 4? 28????? 70?, ????????????4?????????????? B1?B2?B3?B4, ?????? 10?9?9?3??????????????????????????? 1.3  数据处理 ???????????????????????????????????????????????(? 1)?B2? 2????????????????(Amplitude Value)????(Mean Value)??????????????????????????????????? Graphpad Prism 6?????  ? 1  ?????????????? Fig. 1  The Gaussian simulation of larval hatching from egg ribbons of Siphonaria japonica   Marine Sciences / Vol. 41, No. 11 / 2017 69 ????????? SPSS 20.0????????????????????????????????????? -???????????? Kolmogorov-Smirnov ?? , P<0.05 ?????? ; ??????????????????????????????????????????????, P<0.05 ??????; ??, ?????? t?????????????????????????????????????????????????????????????, P<0.05??????? 2  结果 2.1  模拟降水对卵带内胚胎发育的影响 ????????, ?????????????????(? 2)?? 12?36?72? 96 h, ?????????????????????????????????? 69.50%?54.00%?41.50%?61.00%, ??????????? 4 ???????? 65.25%?30.75%?5.50%? 21.25%??????? 96 h?, ?????????????????????????????????? 4.75%?35.00%?21.50%? 17.50%????????????, ??? 12 h ?(P=0.863)??, ??? 3 ????, ???????????????????????????(P<0.01), ????????????????????? 2.2  模拟降水对卵带幼体孵化的影响 ?????????????????? 15?, ???????????????????, ??????????????????? 9 ?? 13??????????????, ??????????????(P=0.05)?????????, ??????????????????????????? t??????, ???????? 20 d???32 d, ???????????????????????(P?? 0.038~0.043)?????, ????????????????????? t????????, ?????????????????????????(P>0.05; ? 1 ?? 2), ??????????????????????????????????  ? 2  ????????????????????? Fig. 2  Development of egg ribbons in the control and treatment groups with simulated rainfall ??**???????????? The double asterisk (**) represents significant differences between the control and treatment (P<0.01)  2.3  自然降水对卵带幼体孵化的影响 ????????, A1?A2?A3?A4??????????????????????????(???? t??, P>0.05; ? 4)??????????????, ????????????????????????????(P>0.05)???????t ??????????????????????????????: ????????????????????????(P>0.05), ?????????????????????????(? 1)?? 70 ????  / 2017?  / ? 41?  / ? 11? ?????????????????????(P<0.05), ??? 1 d??(? 2)????????????, ??????????????????, ???????????, ??????????? 3  讨论 ???????????????, ?????????????????????, ??????????, ???????????????????????????????????[14, 17-18], ?????????????????????????????, ???????????????????????????Eyster[19]???????????????, ????????????  ? 3  ?????????????????? Fig. 3  The effect of simulated rainfall on the larval hatching of Siphonaria japonica ??*??????????? The asterisk (*) represents significant difference between the control and treatment (P<0.05)  表 1  卵带幼体孵化高斯模拟的振幅值统计和独立样本 t 检验结果 Tab. 1  Summary of the Amplitude Values of the Gaussian simulation of larval hatching of Siphonaria japonica ???? t???? ?? ?? ???? n ?????? ??? t? ??? P? ???? ??? 8a 7.808 3.719 ???? ??? ???? 13 7.383 5.984 0.201 18.976 0.843 ???? B1 10 24.578 7.996 ???? A1 ???? 10 35.373 14.433 –2.069 14.049 0.057 ???? B2 9 27.341 8.964 ???? A2 ??? 10 31.451 11.305 –0.882 16.763 0.390 ???? B3 9 31.654 17.933 ???? A3 ???? 10 37.668 20.134 –0.689 17.000 0.500 ???? B4 3 39.677 15.045 ???? A4 ???? 9a 27.370 8.113 1.133 2.401 0.358 a??? 1??????????????  表 2  卵带幼体孵化高斯模拟的高峰值统计和独立样本 t 检验结果 Tab. 2  Summary of the Mean Values of Gaussian simulation of larval hatching of Siphonaria japonica ???? t???? ?? ?? ???? n ?????? ??? t? ??? P? ???? ??? 8a 12.813 1.418 ???? ??? ???? 13 12.976 1.902 –0.209 19.000 0.837 ???? B1 10 7.949 1.344 ???? A1 ???? 10 6.824 0.557 2.442 12.000 0.031 ???? B2 9 7.114 0.458 ???? A2 ??? 10 6.313 0.327 4.345 14.358 0.001 ???? B3 9 7.179 0.825 ???? A3 ???? 10 5.767 0.407 4.652 11.406 0.001 ???? B4 3 5.973 0.108 ???? A4 ???? 9a 5.091 0.693 3.686 9.022 0.005 a??? 1??????????????   Marine Sciences / Vol. 41, No. 11 / 2017 71  ? 4  ???????????????????????? Fig. 4  The effects of natural rainfall on larval hatching at different stages of Siphonaria japonica  ??(?????)??? , ?????????????Woods? DeSilets???[20]??, ???????????????????????, ?????????????????????????Woods ? DeSilets ???[20]??, ???? Melano-chlamys diomedea???????????? 5???????? 0 ?????Pechenik ?[17]?????, ??????? Amphibola crenata????? 5?????????????????????????????? Siphonaria australis ???[18]??, ?????????????? 20 ????????????????????, ????????????????, ??????????????????, ?????????????????? ????????????????????????????, ????????????????????????????????????? 1 d??, ???????????????????????????, ?????????????????????????, ??????? [17]??????? , ?????? Amphibola crenata????? Elysia viridis? 3? Doto???, ????????? 2~3 d ??????????, ????????[19, 21-22]?Pechenik ?[17]?????????????????????(?????)??, ????????????????????????????, ????????????????????????????? Pechenik?[17]???, ??????????????????????????, ????????????????, ??????????????????????????????????????? ??????????????????????????????????????????, ????????????????, ?????????????, ?????????????????????, ???????????????? , ?????????? , ???????????????????[23], ???????????????, ???????????[20, 24]?????????????????????????????????, ????????????????????????(P=0.837; ? 2)?????????????, ????????????????????????????????????????????????????, A. crenata????????????????, ??????10?????????????[17]? ???????????????????? 72 ????  / 2017?  / ? 41?  / ? 11? ??????????????????????????????????, ???????????; ????????????????????, ??????????????????????????????: ????????????????????, ??????????????????????????, ???????????????[10]; ?????????????(?? 50 mm/h), ????????????????, ?????????????????????????????????, ??????????????????????????????????  ? 5  2013??2017?????????????????? 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Cellular responses of encapsulated gastropod embryos to multiple stressors associated with climate change[J]. Journal of Experimental Marine Biology and Ecology, 2010, 383: 130-136. [24] Pechenik J A. Ability of some gastropod egg capsules to protect against low-salinity stress[J]. Journal of Ex-perimental Biology and Ecology, 1982, 63: 195-208. [25] ???. ??? 113a ??????????[J]. ????, 2007, 26(1): 17-25. Wen Zhenzhi. Characters of precipitations in recent 113 years in Xiamen areas[J]. Journal of Oceanography in Taiwan Strait, 2007, 26(1): 17-25. [26] Walther G R, Post E, Convey P, et al. Ecological re-sponses to recent climate change[J]. Nature, 2002, 416: 389-395.   74 ????  / 2017?  / ? 41?  / ? 11? The effects of rainfall on the embryonic development and larval hatching of Siphonaria japonica (Donovan, 1824) WANG Wei1, DING Meng-wen1, DONG Yun-wei1, 2 (1. State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; 2. Xiamen Key Laboratory of Bay Ecological Conservation and Res-toration, Xiamen University, Xiamen 361102, China) Received: Jul. 13, 2017 Key words: Siphonaria japonica (Donovan, 1824); egg ribbons; rainfall; embryonic development; larval hatching  Abstract: As a common intertidal Pulmonata species, Siphonaria japonica (Donovan, 1824) lays its egg ribbons on the intertidal rocky shores. Therefore, rainfall has potential effects on the embryonic development and larval hatch-ing of S. japonica. To investigate the effects of rainfall on the embryonic development and larval hatching of S. japonica, we examined the effects of simulated rainfall on the embryonic development, the cumulative number of larval hatching, and the value and occurrence time of hatching peak in the egg ribbons. We also analyzed the effects of natural rainfall on the cumulative number of larval hatching, the value and occurrence time of hatching peak in different stages of egg ribbons. These results indicated that simulated rainfall had pronounced impacts on the em-bryonic development and larval hatching of egg ribbons (P < 0.05). However, natural rainfall did not affect the cu-mulative number of larval hatching (P > 0.05), but dramatically accelerated the rate of larval emergence (P < 0.05). These results will provide the basic data for understanding the impacts of rainfall on the reproduction and popula-tion dynamics of intertidal gastropods.                                                                         (本文编辑: 康亦兼) 

降水对日本菊花螺胚胎发育和幼体孵化的影响

https://dspace.xmu.edu.cn/bitstream/handle/2288/162504/%e9%99%8d%e6%b0%b4%e5%af%b9%e6%97%a5%e6%9c%ac%e8%8f%8a%e8%8a%b1%e8%9e%ba%e8%83%9a%e8%83%8e%e5%8f%91%e8%82%b2%e5%92%8c%e5%b9%bc%e4%bd%93%e5%ad%b5%e5%8c%96%e7%9a%84%e5%bd%b1%e5%93%8d.pdf?sequence=1&isAllowed=y

降水对日本菊花螺胚胎发育和幼体孵化的影响

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