从福建漳州浯屿岛和漳州浮宫海岸潮间带采集到的10种海藻中分离到66株海藻真菌,采用MTT法对其发酵液乙酸乙酯抽提物的抗肿瘤活性进行检测,发现34株菌株对KB和/或Raji肿瘤细胞具有显著的抑制活性,占供测菌株的52%.其中对KB细胞有抑制作用的菌株有21株,对Raji细胞有抑制作用的菌株有30株,对两种肿瘤细胞均有抑制作用的菌株有17株,分别占活性菌株的62%、88%和50%.实验结果表明,海藻真菌是潜在的抗肿瘤药物资源.Algicolous fungi refer to those fungi which distribute on the surface or inside of algae. Although almost one-third of all known higher marine fungi are associated with algae, algicolous fungi are relatively little investigated. Therefore, in this investigation, we focused on screening algicolous fungi for antitumor, activity and prepare for the future research of bioactive metabolizes. Ten marine algae samples investigated in this study were originated from different intertidal zones of Fujian Province, China. From those algae,sixty-six strains of algicolous fungi were isolated by using selective medium. Those fungi were cultured on PD medium and GPY medium. Organic extracts were screened by the way of MTT( Methyl Thiazolyltetrazolium). As a result,thirty-four strains of the fungi show antitumor activity .The ratio of such active fungi to total offered fungi is 52%. In those fungi, twenty-one strains of fungi demonstrated antitumor activity against KB cell,thirty against Raji cell and seventeen against both KB cell and Raji cell. The ratio of such fungi to total fungi with antitumor activity is 62%,88%and 50% individually. Furthermore, four fungal extracts, namely (G)SCH1,(P)B2, (P)LC13 and(P)LC4, were chosen to study theIC_(50) against Raji cell. The value of them is 80 μg/mL,35 μg/mL,20 μg/mL and 40 μg/mL respectively. Those results indicated that algicolous fungi would be a promossing resourse of antitumor medicament.国家"863"计划(2001AA620401);; 自然科学基金(30070024)资
