Effect of lecithin on the glutathione S-transferase, glutathione pheroidase activitiesand DNA integrity of juvenile Plectorynchus cinctus

Abstract

采用不同卵磷脂添加量的配合饲料投喂花尾胡椒鲷(Plectorhyncchuscinctus)幼鱼,同步测定幼鱼的谷胱甘肽-硫-转移酶和谷胱甘肽过氧化物酶活性以及DNA完整程度。结果表明,谷胱甘肽-硫-转移酶和谷胱甘肽过氧化物酶活性随着饲料中卵磷脂含量的增加而提高,而DNA单链断裂程度随着饲料中卵磷脂含量的增加而减少。这提示卵磷脂能够提高鱼体的抗氧化防御系统的机能。The experiment was conducted to determine the effects of supplemental lecithin in the diet on juvenile Plectorhynchus cinctus. Five isocaloric diets were formulated by the addition of different levels (0,2,4,6,8g/100g diet) of soybean lecithin. The diets were fed to the five groups of juvenile Plectorhynchus cinctus for ten days. The glutathione S- transferase (GST) and glutathione pheroidase (GPx) activities, DNA integrity of juvenile Plectorhynchus cinctus were as- sayed simultaneously. The results indicated that the GST and GPx activities were elevated with increasing of lecithin levels in the diets, the extent of DNA single breaks decreased with the increasing of lecithin levels in the diets. This suggested that lecithin would enhance the function of the antioxidant defend system in the fish.福建省自然科学基金资助项目(139910007

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