STUDIES ON THE CHEMICAL CONSTITUENTS FROM SEA CUCUMBER MENSAMARIA INTERCEDENS Ⅳ ISOLATION,PROPRETIES AND ANTITUMOR ACTIVITY OF THE GLYCOPROTEIN FROM MENSAMARIA INTERCEDENS
二色桌片参〔Mensamaria intercedens( L ampert)〕的体壁经水煮提、分离、最后经 Sephadex G- 2 0 0柱层析纯化得二色桌片参糖蛋白 ( GPMI- )。GPMI- 经酶解后得二色桌片参糖蛋白 ( GPMI- )。分别研究了GPMI- 和 GPMI- 的性状、化学组成和抗肿瘤活性 ,结果表明 :GPMI- 和 GPMI- 都是糖蛋白 ,但蛋白组成和糖含量明显不同。GPMI- 对小鼠 S180 肉瘤有明显抑制作用 ,而无直接毒性 ,抑瘤率为 4 8.8%。而 GPMI- 却能显著提高 S180 肉瘤荷瘤小鼠的脾指数 ,呈免疫增强作用。Glycoprotein of Mensamaria intercedens(GPMI Ⅰ) was extracted from the body of Mensamaria intercedens with hot water,and purification was finished with column chromatography on Sephadex G 200. GPMI Ⅰ was hydrolyzed with enzymes to obtain glycoprotein of Mensamaria intercedens Ⅱ(GMI Ⅱ).The chemical property,composition and antitumor activity of GPMI Ⅰ and GPMI Ⅱ were studied. The results showed that GPMI Ⅰ and GPMI Ⅱ were two kinds of glycoproteins in which the content of protein and sugar were different. GPMI Ⅱ could significantly inhibit the growth of Sarcoma 180 cells implanted subcutaneously in mice (P<0.05) at the dose of 20 mg·kg -1 per day ×10 with no sign of toxicity. The ratio of tumor inhibition is 48.8%. On the other hand, GPMI Ⅰ increased the weight of spleen of mice with S 180 cells at the dose of 30 mg·kg -1 per day×10.福建省重中之重项目资
