氢作为一种清洁的能源引起了人们的普遍重视.实验以产酸克雷伯氏菌(Klebsiella oxytoca)HP1为产氢菌株,以稻草粉为产氢底物,进行同步糖化发酵(Simultaneous Saccharification and Fermentation,SSF)产氢.对影响同步糖化发酵产氢的单因子进行试验,选取对氢产率影响较大的因子:温度、pH、纤维素酶用量等进行L9(34)正交试验.结果表明同步糖化发酵产氢的最佳条件为:温度40℃,pH6.5,纤维素酶用量为20FPAU/g稻草粉,摇床转速100r/min,发酵时间42h.在该条件下的最大氢产率为110.6mL/g稻草粉,稻草粉的氢转化率为22%.进行了10L放大发酵产氢试验,最大氢产率为122.3mL/g稻草粉,氢转化率为24.3%.与分步糖化发酵(Separate Hydrolysis and Fermentation,SHF)产氢相比,氢产率提高34.4%.研究表明,利用同步糖化发酵工艺可以提高生物制氢的产量和得率.Being regarded as a clean energy source,biological hydrogen attracts more and more attention.Hydrogen production from rice straw by simultaneous saccharification and fermentation (SSF) was studied using the Klebsiella oxytoca HP1 in this report.The experiments of batch ferment hydrogen production were carried out in 600 mL serum bottles.Single factor and orthogonal experiments showed that the optimal conditions for hydrogen production were:pH6.5,ferment temperature 40℃,cellulose dose 20 FPAU/g rice straw,revolution 100 r/min and ferment time 42 h.The maximum H2 production (110.6 mL/g rice straw) and H2 yield efficiency (22%) were obtained under the above conditions.The enlarge experiments were carried out in a 10 L bioreactor,a maximum H2 production of 122.3 mL/g rice straw and a H2 yield efficiency of 24.3% were achieved.The H2 production increased by 34.4% compared with the separate hydrolysis and fermentation hydrogen production.This research indicated a perfect application of SSF in biohydrogen production from cellulose material.国家自然科学基金项目(30470395);; 厦门市科技项目(3502Z20041070);; 厦门大学新世纪优秀人才计划项目(X07114)资
