利用转基因植物生产疫苗是目前植物基因工程的一个研究热点,具有生产简单、成本较低、使用安全方便、易贮存等优点.本实验通过农杆菌介导的遗传转化,以O型和A型口蹄疫抗原决定簇基因O21O14A21转化胡萝卜.通过筛选,获得潮霉素抗性愈伤组织,经胚状体再生得到156棵抗性苗.通过GUS染色检测,GUS报告基因在部分转化的愈伤组织中瞬时表达,并在部分抗性苗中稳定表达.对部分抗性植株进行PCR和PCRSouthern杂交检测,证实目的基因已经成功整合到胡萝卜基因组中.Vaccines derived by transgenic plants are becoming one focus of plant gene engineering, which has such advantages as easy production,low cost,convenience and safe use and easy storage.Fused genes O_21 -O_14 -A_21 containing both type O and A FMDV antigenic determinant were introduced into carrot mediated by Agorbacterium.Hygromycin-resistant carrot callus were obtained by the medium containing antibiotic.156 Hygromycin-resistant seedling individuals were regenerated and selected from the embryoid of the above carrot callus.GUS expression test showed that GUS reported genes were transitorily expressed in some of transformed callus and stably expressed in some of Hygromycin-resistant seedlings.The results of PCR and PCR-Southern blotting showed that the O_21 -O_14 -A_21 gene was successfully integrated into the genomes of carrot plants.国家科技部转基因专项;; 福建省科技厅重点项目资
