为研究锯缘青蟹对常见重要病原菌的急性反应蛋白质组,将锯缘青蟹随机分为四组,分别注射生理盐水、副溶血弧菌、鳗弧菌和嗜水气单胞菌.继而提取肌肉蛋白进行双向电泳,通过比较各组肌肉蛋白的双向电泳图谱获得三个差异表达的蛋白.对这三个差异蛋白进行肽质量指纹图谱及其生物信息分析,鉴定为Calexc itin、无翅蛋白片断、速激肽相关肽.这些结果对研究锯缘青蟹的抗病蛋白及其机理具有重要意义.Innate immunity system provides the body with its first line of protection against external injury.A acute phase response (APR),as a important part of it,has been paid attention to.Proteins involved in APR are called as acute phase protein (APP).Present studies indicated that proteomics is an ideal tool to characterize APP.In the current study,APP profiles were investigated in the muscle of Scylla serrata against Vibrio parahaemolyticus,Vibrio anguillarum and Aeromonas hydrophila with the use of proteomic methodologies.For this purpose,Scylla serrata were separately injected with the three pathogenic bacteria and 0.85% brain was used as a control.Samples were prepared from the muscle at the fifteen minutes following the injections.Then the proteins were separated by two-dimensional gel electrophoresis (2-DE).Three proteins with variances were achieved by comparison between the 2-DE maps.With the use of matrix-assisted laser dsorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) and bioinformatics,the three variances were elementarily identified as a protein similar to calexcitin,wingless (Fragment) and tachykinin-related peptide.Calexcitin,a calcium sensor protein,can bind calcium and GTP,inhibit potassium channels,and enhance membrane excitability.Wingless belongs to Wnt family,which is a signal molecule on embryonic development and cancer.Tachykinin,a neuropeptide,plays an important role in regulation of carbohydrate metabolism,muscle contractility and pigment moving etc.Interestingly,there has been no report on the relationship between the three proteins and APR.Our results,therefore,provide valuable experimental evidences for approach to APR in a crab.国家“863”计划(2002AA603013
