根据转基因农作物中最常用的花椰菜花叶病毒启动子 (CaMV 3 5S)和根癌农杆菌终止子(NOS)的序列 ,设计合成了两对不同的引物和相对应的两种荧光双链探针 (FDCP) ,分别建立了多重PCR、应用FDCP的实时荧光PCR同时检测转基因成分 3 5S启动子和NOS终止子的方法 .并利用该套方法对马铃薯、大豆、玉米、甜椒、番茄等实物样品进行了检测 ,发现 1 3份样品中有 6份检出3 5S启动子、NOS终止子 ,其余 7份样品的检测结果为阴性 .表明作者建立的多重PCR方法能有效检测出 3 5S和NOS成分 ,其中多重PCR法具有灵敏度高、特异性好的特点 ,多重荧光PCR法则更为简便、快速、准确The article is to establish a PCR method for detecting transgenic component 35S promoter derived from Cauliflower Mosaic Virus and NOS terminator derived from Agrobacterium tumefaciens simultaneously. According to the specific sequence of 35S and NOS which have been used in transgenic crops frequently, two pairs of primers and two pairs of corresponding fluorophore double chain probes(FDCP)were designed and synthesized. PCR & Fluorescence PCR(FPCR)methods were established for general screening of transgenic component 35S and NOS simultaneously with Multiplex PCR in a tube. 13 samples were tested with PCR & FPCR. The results showed that 6 samples were positive, 7 samples were negative. The methods gave a sensitive, specific, simple and accurate detection of transgenic component, and thus provided a useful tool for routine analysis of raw and processed food products.厦门市科技计划项目 (350 2Z2 0 0 1 1 0 9)资助课
