[目的]了解HBV携带者血清HBV-NRAg的检出情况,探讨HBV感染后HBV-NRAg与HBVM、HBVDNA载量的相关性。[方法]连续收集了从业人员的体检标本(n=4968),ELISA法检测血清HBV-NRAg和HBVM,同时以FQ-PCR检测核酸指标并辅以分区套式PCR和DNA测序作为证实检测,统计分析HBV-NRAg与HBVM、HBVDNA的相关性。[结果]HBV-NRAg的阳性率7.5%,略低于HBsAg的8.3%,略高于HBV DNA的6.8%。在HBV携带者中HBV-NRAg检测与HBV DNA的符合率87.4%,高于HBsAg或HBeAg,其S/CO值与HBVDNA载量线性相关(R2=0.927)。通过HBV-NRAg的检测筛选到6例HBV隐匿性感染,其中3例存在S区"a"基因突变。[结论]对于HBV感染后病毒复制状态和传染性的评价,HBV-NRAg可以替代HBV DNA作为良好的评判指标,而且可以用于变异毒株的筛选。[Objective]To investigate detection situation of HBV-NRAg in serum from HBV carriers,and discuss the correlation of the HBV-NRAg with HBVM and HBVDNA loads,respectively.[Methods]4968 serum samples were consecutively collected from the health examination of service workers,and HBV-NRAg and HBVM were detected by using ELISA.At the same time,the nucleic acid index of HBV DNA was examined by FQ-PCR,supplemented with detecting nested-PCR and DNA sequencing as certified tests.Statistical analyses were performed to analysis the correlation of HBV-NRAg with HBVM and HBVDNA,respectively.[Results]The total positive rate of HBV-NRAg was 7.5%,which was lower than HBsAg(8.3%) but higher than HBV DNA(6.8%) .A good conformability was observed between the test results of HBV-NRAg and HBV DNA.Meanwhile the S/CO values was closely correlated with HBV DNA loads(R2=0.927) .Furthermore,6 cases of concealed HBV infection were screened out by HBV-NRAg test,in which HBV genome had a point mutation in the‘a’epitope of S region.[Conclusion]As a replacement of HBV DNA,HBV-NRAg can be seen as a good evaluation index in terms of the evaluation on replicating phase HBV and its infectivity after being infected.Moreover,it also can be applied to effective screening of HBV mutation.厦门市重大疾病科研攻关项目(WKZ0501
