以一株H5N1亚型禽流感病毒RNA为模板,用RT-PCR方法,扩增M基因全长,将PCR产物克隆于pMD18-T载体,测序结果表明所克隆的982个核苷酸的片段包含了M1和M2基因的完整阅读框架,通过软件推导M1和M2基因分别编码252和97个氨基酸,将M全长序列与Genbank收录的10株H5N1亚型流感病毒M基因序列进行比较,病毒株之间M基因核苷酸序列同源性为92.3%~99.3%,编码的两个蛋白M1和M2氨基酸序列间同源性分别为为96.0%~98.8%和92.9%~99%,分子进化分析揭示了病毒株间的亲源关系.The matrix(M) gene was amplified from an H5N1 subtype avian influenza virus(AIV) by RT-PCR.The complete M gene was exactly cloned into pMD18-T vector.The results of sequence showed that the 982 bp fragment covered complete open reading frame of M1 and M2 gene,and M1 and M2 gene coded 252 and 97 amino acid,respectively.The results of comparative sequence analysis indicated that the nucleotide sequence of M gene of isolate shared 92.3%~99.3% homology with M gene of other 10 strains H5N1 AIV,and putative amino sequence M1 gene and M2 gene shared 96.0%~98.8%and 92.9%~99% homology with other 10 strains.The phylogenetic analysis of M1 and M2 gene revealed the relationships among 11 strains H5N1 AIV.福建省“畜牧业健康养殖关键技术研究与示范”重大专项;; 国家基础科学人才培养基金项目(J0603649)资
