利用60Co-γ射线对坛紫菜(Porphyrahaitanensis)原生质体进行辐照诱变,发现原生质体对60Co-γ射线有极强的耐受性。前期的培养结果表明,原生质体是一种理想的诱变材料。诱变结果表明,60Co-γ射线对坛紫菜可诱变剂量范围很大,从200~1300Gy均有突变株生成。诱变后细胞的存活率不宜作为坛紫菜诱变最佳剂量的指标。60Co-γ ray was used to induce mutant on protoplast of Porphyra haitanensis.The results showed that the protoplasts of P.haitanensis could survive under a wide range of γ-rays.Primary culture showed that the protoplast was suitable for mutagenesis.Further results also showed that mutants could be induced in a very wide range of dosages of 60Co-γ ray.The mortality was not a suitable parameter for the evaluation of the mutant-induction efficiency in P.haitanensis.国家863计划项目(2002AA603023);; 福建省重大农业科技项目(2001Z017
