通过滤纸片法初步测定了黄连和乌梅水提液的抗菌性能,用比浊法进一步考察了它们对5种肠道菌体外生长的影响。结果表明,0.001 g/mL黄连和乌梅水提液均能够有效抑制产气荚膜梭菌和金黄色葡萄球菌的生长,轻微促进干酪乳杆菌的生长,但对嗜酸乳杆菌的生长只有微弱的抑制作用。0.01 g/mL黄连水提液对大肠杆菌的生长影响不大。而0.001 g/mL乌梅水提液可促进大肠杆菌的生长,但当质量浓度升高至0.005 g/mL时,则对大肠杆菌的生长表现出抑制作用。因此,黄连、乌梅水提液可能是潜在的益生元,是功能性食品配料、绿色饲料添加剂的候选者。The effects of aqueous extracts from C.chinensis Franch root(Coptis root)-derived and P.mume fruit-derived materials on the growth of intestinal bacteria were examined firstly by using impregnated paper disk method,and further by using spectrophotometric method.Both Coptis root and P.mume extracts exhibited potent inhibition against Clostridium perfringens and Staphylococcus aureus at mass concentration as low as 0.001 g/mL,whereas only weak inhibitory activity was detected against Lactobacillus acidophilus.Moreover,growth of Lactobacillus casei was slightly promoted by both extracts at the same mass concentration.No inhibition against Escherichia coli was observed from treatment with Coptis root extracts even at 0.01 g/mL.On the contrary,P.mume extracts showed slight stimulation effects on the growth of E.coli at 0.001 g/mL,but exhibited significant growth inhibition at 0.005 g/mL.These results indicated that Coptis extracts and P.mume extracts may be potential prebiotics,and could be alternatives for functional food additives and green feed additives
