对休眠的巨大芽孢杆菌(Bacillusmegatherium)D01菌体吸附Pt4+的作用过程进行了表征.TEM和XPS展示Pt4+沉积的主要部位是菌体细胞壁并在其表面逐步被还原为Pt0.FTIR表明D01菌体细胞壁肽聚糖层肽链上的酰胺键、肽链侧链的氨基酸残基离子化羧基以及糖类化合物的羟基为吸附Pt4+的活性基团;肽聚糖层部分多糖的水解产物还原糖,其游离态的醛基为电子供体,将Pt4+原位还原成Pt0.Some characteristics of Pt4+ biosorption by resting cell of Bacillus megatherium D01 biomass have been reported. TEM micrograph and XPS spectra of platinumloaded D01 biomass showed that platinum deposited on the cell and revealed that Pt4+ was reduced to Pt0. FTIR spectra demonstrated that active groups such as amido bond, ionized carboxyl group of remnant aminoacid and hydroxyl group of saccharides from the peptidoglycan layer on the cell wall seem to be the sites for Pt4+binding; free aldehyde group from reducing sugars, i.e., hydrolysates of polysaccharides on the peptidoglycan layer, serving as the electron donor, in situ reduced Pt4+to Pt0.国家基础研究发展规划(001CB108906);; 自然科学基金(29876026,29743001)资
