Gas Chromatographic Analysis of Fatty acid in Microalgae

Abstract

以正十九酸(C19:0)作内标,藻体经超声萃取、浓缩吹干,进行抽提酯化后采用毛细管气相色谱法分析测定。该方法重现性好,相对偏差为0.53%~5.49%,回收率较高,为95.1%~102.5%,检测限为0.0072 μ g。In this study, a convenient and rapid method based on capillary gas chromatography is established for the analysis of fatty acid in marine microalgae. By this method, the fatty acids in algal cells are extracted by the ultrasonic method, then concentrated and desiccated in nitrogen gas. After esterification, the fatty acids are detected by capillary gas chromatography .The result shows the high recovery ratio (95.1%~l02.5%), the low relative deviation (0.53%~5.49%) and the low detecting threshold (0.0072 μg) of this method.国家自然科学基金(No.39870565);; 福建省自然科学基金(No.C97006)共同资

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