采用PHA结合秋水仙素活体注射,取条纹斑竹鲨(Chiloscyllium plagiosum)脾脏经低渗处理,空气干燥制片法制作染色体标本,对染色体进行Giemsa染色,完成条纹斑竹鲨染色体核型分析.研究结果表明,条纹斑竹鲨的染色体组由20对中部着丝粒染色体、12对亚中部着丝粒染色体、5对亚端部着丝粒染色体和14对端部着丝粒染色体构成,染色体核型公式为:2n=102=28t+10st+24sm+40m,染色体臂数NF=166,其中1对亚中部着丝粒染色体带有明显的次缢痕.与其他软骨鱼类相似,条纹斑竹鲨同样具有数目众多的染色体二倍体数,但以中部着丝粒和亚中部着丝粒染色体为主.The chromosome specimens of the lip shark Chiloscyllium plagiosum were obtained from the spleen cell by vivo injection of PHA and cultivation of colchicines,hypatoic-air drying technique,and then,by studying their Giemsa stain,the analysis of the karyotype was completed.According to the chromosome centromeric index values found,the karyotype of C.plagiosum is composed of 20 metacentric,12 metacentric-submetacentric,5 telocentric-subtelocentric and 14 telocentric pairs,and the karyotype formula is:2n=102=28t+10st+24sm+40m.NF=166,and there was one pair of metacentric-submetacentric chromosomes with secondary constriction.Compared to the other cartilaginous fishes,C.plagiosum had high diploid chromosome number,most of which were metacentric and metacentric-submetacentric chromosomes.国家自然科学基金(40776083,40876080);; 厦门市科技项目(3502Z20063022)资
