采用超声破碎协同蒸馏水提取海水小球藻(Chlorella)中的多糖,多糖经除蛋白、柱纯化、水解和乙酰化等一系列步骤后进行气相色谱分析,得到该多糖的单糖组成为:鼠李糖、核糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖,其含量分别为3.0％、1.9％、1.3％、1.3％、1.5％、82.8％和8.2％。结果表明该法精密度高,相对标准偏差为2.1％～6.4％,回收率为96.2％～102.1％,检测限为0.002 38μg。The ultrasonication associated with extraction in. distilled water was used to extract polysaccha-ride in Chlorella. The crude polysaccharide was treated with a series of procedures of protein-removing, purification with column, hydrolysis and acetylation, then was analysized by Gas Chromatography to gain the saccharide components which are rhamnose、 ribose、 arabinose、 xylose、 mannose、 glucose and galacose and their percentage are 3. 0% ,1. 9% ,1. 3% ,1. 3%,1. 5% ,82. 8%and8. 2% respectively. The experimental result shows that the precision of the analytical method is good, with a relative standard deviation of 2. 1%-6. 4%. The recovery of the method is 96. 2%-102. 1%, and the detection limit is 0.002 38 μg.国家自然科学基金(39870565
