应用PCR技术扩增了我国南方重要养殖石鲈科鱼类斜带髭鲷(Hapalogenys nitens)和花尾胡椒鲷(Plectorhinchuscinctus)线粒体DNA(m tDNA)的16S rRNA基因片段,纯化后分别进行EcoRⅠ、M seⅠ、PstⅠ、SacⅠ和HindⅢ等5种限制性内切酶酶切和测序分析.酶切结果为:斜带髭鲷16S rRNA扩增片段被M seⅠ和SacⅠ两种内切酶酶切,花尾胡椒鲷16SrRNA扩增片段只被M seⅠ酶切.两者在M seⅠ和SacⅠ酶切图谱的差异可作为区分两者的遗传标记.16S rRNA序列分析结果表明,斜带髭鲷和花尾胡椒鲷的遗传相似度为86.94%,遗传差异为13.06%.进化上,两者分化年代大约为1.98×106年前.The fish Hapalogenys nitens and Plectorhinchus cinctus were both valued reared species in family Pomadasyidae.The mitochondrial 16S rRNA gene fragments of two fish were both amplified by polymerase chain reaction(PCR),sequenced and digested with five restriction enzymes of EcoR Ⅰ?Mse Ⅰ?Pst Ⅰ?Sac Ⅰand Hind Ⅲrespectively.The results showed that DNA sequences about 680 base-pair(bp) of 16S rRNA in two species were acquired.The fragment of 16S rRNA gene in P.cinctus was only digested by Mse Ⅰ,and the fragment in H.nitens was digested by Mse Ⅰand Sac Ⅰ.And the number of cleavage sites of P.cinctus digested by Mse Ⅰwas different from H.nitens.These differences could be used as the genetic marker to distinguish P.cinctus from H.nitens.Aligning of the DNA sequence of 16S rRNA gene revealed that genetic similarity and the genetic difference between two species were 86.94% and 13.06% respectively.And the 16S rRNA sequence divergence indicated that divergence of the two species occurred about 1.98×106 years before.国家自然科学基金(30070595);; 福建省自然科学基金(B80010003)资
