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钉螺体内细菌群落结构pcrdgge分析方法的建立
Authors
余育和
倪加加
胡合华
Publication date
1 January 2010
Publisher
Abstract
建立了通过PCR-DGGE研究钉螺体内细菌群落结构的方法,并采用此技术分析了钉螺体内细菌群落结构。钉螺样本采自湖北省荆州市,样本带回实验室经破壳解剖后取清洗的钉螺内脏放入无菌离心管后经酚-氯仿法抽提微生物DNA。使用获得的微生物DNA为模板,选择细菌原核通用引物F357-GC和R518进行PCR扩增得到大约250bp的目的片段,然后采用变性梯度凝胶电泳结合DNA测序技术对获得的目的片段进行分析。结果显示此技术能够区分幼螺和成螺体内细菌群落结构差异,并发现在成螺体内有大量的Pseudomonas属和Acidobacteria属细菌出现。</p
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Institute of Hydrobiology, Chinese Academy Of Sciences
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oai:ir.ihb.ac.cn:342005/36336
Last time updated on 08/06/2020