Geldhof, Peter

Heda, Mio Ayana

Levecke, Bruno

Medebo, Daniel Dana

Mekonnen Kurmane, Zeleke

Timire, Collins

Tweya, Hannock

Verdonck, Kristien

Vlaminck, Johnny

Vogt, Florian

English

Ghent University Academic Bibliography

  The clinical sensitivity of standard direct wet mount microscopy for soil-transmitted helminth infections in school children in Jimma, South-West Ethiopia   Daniel Dana1,3, Johnny Vlaminck2, Zeleke Mekonnen1, Mio Ayana1, Florian Vogt3, Kristien Verdonck3 , Hannock Tweya4, Collins Timire4, Peter Geldhof2, Bruno Levecke2  1School of Medical Laboratory Science, Faculty of Health Science, Institute of Health, Jimma University, Jimma, Ethiopia 2Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium 3Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium  4The International Union against Tuberculosis and Lung Disease, Paris, France  Abstract  Introduction: Soil-transmitted helminthiasis is a disease caused by Ascaris lumbricoides, Trichuris trichiura and the two hookworms (Ancylostoma duodenale and Necator americanus) and remains a major public health problem in school children in Ethiopia. Although direct wet mount microscopy is the standard means to diagnose intestinal parasitic diseases in health care facilities in Ethiopia, it remains unclear what its diagnostic performance is for soil-transmitted helminths .  Aim: To determine the clinical sensitivity of standard direct wet mount microscopy for soil-transmitted helminth infections compared to the composite reference standard in school children in Jimma, South-West Ethiopia  Methods: A school based cross-sectional study was performed in Jimma Town and included 600 children from 10 primary schools. The clinical sensitivity of direct wet mount microscopy was compared to a composite reference standard consisting of Kato-Katz thick smear, McMaster and Mini-FLOTAC. Results:  The composite reference standard about 210 Ascaris (35.0%), 312 Trichuris (52.0%) and 102 hookworm cases (17.0%). The median intensity of infections (expressed as eggs per gram of stool (EPG)) equalled 2,057 EPG for Ascaris, 200 EPG for Trichuris and 110 EPG for hookworms. The sensitivity of direct wet mount microscopy was 73.8% for Ascaris, but was only around 17% for both Trichuris and hookworms. For each of the three soil transmitted helminths there was an increase in sensitivity as a function of increasing intensity of infections. For Ascaris, the sensitivity ranged from 30.2% for the lowest infection intensities to 94.3% for the highest levels of infection intensity. For both Trichuris and hookworms, the sensitivity ranged from nearly 0% to 42.9% across the different levels of infection intensity. Conclusion: Using direct wet mount microcopy severely underestimated the prevalence of soil transmitted helminths. The sensitivity of direct wet mount microcopy is low for STH, particularly for infections with Trichuris and hookworm when the infection intensities are low.  

The clinical sensitivity of standard direct wet mount microscopy for soil-transmitted helminth infections in school children in Jimma, South-West Ethiopia

https://biblio.ugent.be/publication/8663496/file/8663497.pdf

The clinical sensitivity of standard direct wet mount microscopy for soil-transmitted helminth infections in school children in Jimma, South-West Ethiopia

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Ghent University Academic Bibliography