Hemker, H.C.

Loeliger, E.A.

van Halem-Visser, L.P.

English

Maastricht University Research Portal

   Plasma free of factors II, VII, IX, and XCitation for published version (APA):Loeliger, E. A., van Halem-Visser, L. P., & Hemker, H. C. (1973). Plasma free of factors II, VII, IX, and X:Need for high speed centrifugation after Al(OH)3 absorption . Thrombosis et diathesis haemorrhagica,29(1), 211-212.Document status and date:Published: 01/01/1973Document Version:Other versionPlease check the document version of this publication:• A submitted manuscript is the version of the article upon submission and before peer-review. There canbe important differences between the submitted version and the official published version of record.People interested in the research are advised to contact the author for the final version of the publication,or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and pagenumbers.Link to publicationGeneral rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyrightowners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with theserights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain• You may freely distribute the URL identifying the publication in the public portal.If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license above,please follow below link for the End User Agreement:www.umlib.nl/taverne-licenseTake down policyIf you believe that this document breaches copyright please contact us at:repository@maastrichtuniversity.nlproviding details and we will investigate your claim.Download date: 04 Jan. 2021Sonderdruck8s?'Tirage À partE D I T O R :  D I t .  R O S E I l Á l t Y  B I G c s ,  O X F O I È DÁ S S O C I A T E  E D I T O I È S :  Q .  l r .  R I Z Z L .  O X F O I t D .  A ] { D  F .  D U C K D R T .  B A S E Lvol,. xxlx 28. II. 1973Plasma FreeNeeil for High SpeeilLetter to the Editorof Factors [[. VI[, IX, and XCentrifugation after Al(OH)BAbsorptionF"  K.  SCITATTAUER VER,LAG ,  STUTTGAR,T-NEW YORK. ','. ,,1*ïi ,Dear Madam zgth Decemb er, lg72The procedure to remove the vitamin K-dependent clotting factors from citratedplasma (either normal or from patienrs;;;il;;;;;;?s"d"ts) has been wellstandardized with respect to. the type u erroui, ilb" ""rd"ËtfrJfu,rutio' rnade bv"British Drus House*-" h?l u"* í*a,lóo g "r'i,nilr od;lïJËil;"il-ffiï Jrdistiled wareil, rhe rime or i"""Ë;;;;;ilf" il;;;;#;1 ;#; adsorption rakesplace (Biggs, lg72)- Theimportance of stanáardizing the centrifugation step as well,is insufficiently realized, hoiever.An apparently clear supernatant is obtained after a 2.500-9.000 rpm. run for r0 min.at room t'emperature' yhigh_suggests complete removar rr artorrïr. -[rowever, if thesupernatant thus obtained (low-speed supàrnatant) is used for the-construction oÍ athrombotest reference curve (Fig. 1), "d;;;u obíained, *bi"i;;";;ffi;ï;Ë#| 5 lo ls oiruti2olx'ig' 1 correlation between coagulation time (Thrombotest) and dilution of normal plasma inAl(Oll)o-adsorbed plasma., I : "i1ifirql i.."*"f fi"*-" ; 2:ll2 dilution etc. The lowor curvesare obtainod from normal pt".::".*1".*d rn high-sËed supornaf&nt, o-1 with not incubateddilutiorx' and o-----o with dilution" l',c,rratàa tor B hours at room lom'erature. The upporcnrvos aro obtained from dilutions in low_spood.supornetant; f i irnmodiately, andn-____ D 3 hours after dilutions had be.n:pJpa.ed. -ooÈCqooïhronbos. Diathes. haemorrh. (Stuttg.;,.-* -? ,_ -_-:---*_,--*:>i:ia,;:;.-- _ii,:-!4::_iii,.,i::glJ,-::4ÍtË:.iq-,., _.iÈ*i1:Laf::+,3;{:-*a,Êi..asÈ;::.-:..:!-:::rr:+:,:F2t2different from those obtained with saline or buffer (the reference curve given by theproducer of thrombotest), but which are also far f".; ;;ubl" ;láigor*tio' times of thediluted plasma samples are considcrably longer after three hours incubation at roomtemperature than immediately after tle preparation of the dilutions. rn contrast,with supernatant obtained aftLr centrifugitio'n ,t z0^0óó;ï" B, min. at 4"c (hish-speed supernatant) the cur'e parallels thË producer,u ,uiirr-" o" uïtr;;;";;"; Àd;;-dant of the time of incubatiori of the dilutiàT Htch:-pJrirpJàuruor, therefore, isthe oniy preparation suitable for use m u dilor.ri ir.ïh"-";;";ructions of referencecurves.The differences in coagulation times between low-speed and high_speed supernatantseen in x'ig. 1 are most easily explained ,r "u,o*uJny "-uÀi."1- Ëiïrtorr), still presentin the low-speed supernatant anà adsorbing, ata retuti,r"ry to* ""te, tactors rr, vrr, rx,and X from the dn;tud normal plasma. i"-J""a.1 smail pelret of Ar (oH), can be Íoundat the bottom of the tube af!e1 high-speed centrifugation of the iow-speed supernatant.when reference curves of thrJmboplastins othei than Thrombotest are considerod, ÍÉ;:the influence of al(oH)3 remnants i.'^o"h less obvious. trr-i.ïá"."d partly by t6" ',.."."",lower sensitivitv oi mosl of the other thromboplastins, partly by a factor vrr- orfactor X-like aciivitv often present irr;;;r"y tissue thromboplastins. This might bethe reason why the eïïect has'escap"Jtn"ïu.".oer,s eye for sucli a long time., Sincernany investigators still iefer to percentages obtainec from reference curvesobtained from d'utions of normal prasmà rÀ.itl 1ii(oH), ,a**ào plasma (Leadingffriï;i,|I'), the importance of sraridardizilg 11r" "errt"f,igurion ri"p rt a high g valueThe adverse effects of not recognising al(oH), remnants already haunt the ritera-ture: for instance the Equivarent ï,atio"MetÀoa á"".o.ai"gi9ïïgi. ,ra Denson (r967)for thromboplastin standardization quite recently has undergone a criticism (Litteland Ratnofl 1g6g) which, rooking at ïhe curoes on which the ïriticism is based (page143 and 144 ol this reference), áust be based on the o.ru*r""**u of the afteraetdescribed in this communicatioí. But how many other misunderstandinss do exist ?S,incerely,X). A. Loeliger,L. P. aq,n Halem-Visser.' H. C. HëmkerH aematology B ection anilSectíon ol Bíochemistru I I,Department ol Internal Med,icineUniuersity Hospital,Leid,en, The N etherland,sReferencesBrecs, R" (1972\: Hum:tn Blocd' coaguloti,on, Ha,emostasis a,nil Thrombtsrls. Illackwell scientificPublications, Oxford.Lnloruc Anrrcr,o (1g69):_Con-trol of anticoagulant therapy. Lancot, g, 990,B rccs 'R 'andDnrso r * 'K 'w '8 . ( 1967 ) :  s i anda rd i za t i à í " i t r r " o r " - " r r gep ro th romb in t imo fo r_ the control of articoagulart, therapy. British Medical Journal, -1, g4.Lrrrnr" a' s' and Rarrvorr, o-. o. litiool r A cornparative study of several óhromboplastins used toi"rË."" the one-stage prothrombin time. Thrombosis et diatho"i" nae*o""na!ica suppl. 85,Roceived for publicaíion 14th Decembe r. 197 2.t?{i"',tj:,1í,*,,ï; lffiï";rl:."t:r;1,ï:::-. - Der vertag behàlt sidr aue Reóte, besonders die des Nadrdrud<es,

Plasma free of factors II, VII, IX, and X:Need for high speed centrifugation after Al(OH)<sub>3</sub> absorption 

   Plasma free of factors II, VII, IX, and XCitation for published version (APA):Loeliger, E. A., van Halem-Visser, L. P., & Hemker, H. C. (1973). Plasma free of factors II, VII, IX, and X:Need for high speed centrifugation after Al(OH)3 absorption . Thrombosis et diathesis haemorrhagica,29(1), 211-212.Document status and date:Published: 01/01/1973Document Version:Other versionPlease check the document version of this publication:• A submitted manuscript is the version of the article upon submission and before peer-review. There canbe important differences between the submitted version and the official published version of record.People interested in the research are advised to contact the author for the final version of the publication,or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and pagenumbers.Link to publicationGeneral rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyrightowners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with theserights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain• You may freely distribute the URL identifying the publication in the public portal.If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license above,please follow below link for the End User Agreement:www.umlib.nl/taverne-licenseTake down policyIf you believe that this document breaches copyright please contact us at:repository@maastrichtuniversity.nlproviding details and we will investigate your claim.Download date: 01 Oct. 2023Sonderdruck8s?'Tirage À partE D I T O R :  D I t .  R O S E I l Á l t Y  B I G c s ,  O X F O I È DÁ S S O C I A T E  E D I T O I È S :  Q .  l r .  R I Z Z L .  O X F O I t D .  A ] { D  F .  D U C K D R T .  B A S E Lvol,. xxlx 28. II. 1973Plasma FreeNeeil for High SpeeilLetter to the Editorof Factors [[. VI[, IX, and XCentrifugation after Al(OH)BAbsorptionF"  K.  SCITATTAUER VER,LAG ,  STUTTGAR,T-NEW YORK. ','. ,,1*ïi ,Dear Madam zgth Decemb er, lg72The procedure to remove the vitamin K-dependent clotting factors from citratedplasma (either normal or from patienrs;;;il;;;;;;?s"d"ts) has been wellstandardized with respect to. the type u erroui, ilb" ""rd"ËtfrJfu,rutio' rnade bv"British Drus House*-" h?l u"* í*a,lóo g "r'i,nilr od;lïJËil;"il-ffiï Jrdistiled wareil, rhe rime or i"""Ë;;;;;ilf" il;;;;#;1 ;#; adsorption rakesplace (Biggs, lg72)- Theimportance of stanáardizing the centrifugation step as well,is insufficiently realized, hoiever.An apparently clear supernatant is obtained after a 2.500-9.000 rpm. run for r0 min.at room t'emperature' yhigh_suggests complete removar rr artorrïr. -[rowever, if thesupernatant thus obtained (low-speed supàrnatant) is used for the-construction oÍ athrombotest reference curve (Fig. 1), "d;;;u obíained, *bi"i;;";;ffi;ï;Ë#| 5 lo ls oiruti2olx'ig' 1 correlation between coagulation time (Thrombotest) and dilution of normal plasma inAl(Oll)o-adsorbed plasma., I : "i1ifirql i.."*"f fi"*-" ; 2:ll2 dilution etc. The lowor curvesare obtainod from normal pt".::".*1".*d rn high-sËed supornaf&nt, o-1 with not incubateddilutiorx' and o-----o with dilution" l',c,rratàa tor B hours at room lom'erature. The upporcnrvos aro obtained from dilutions in low_spood.supornetant; f i irnmodiately, andn-____ D 3 hours after dilutions had be.n:pJpa.ed. -ooÈCqooïhronbos. Diathes. haemorrh. (Stuttg.;,.-* -? ,_ -_-:---*_,--*:>i:ia,;:;.-- _ii,:-!4::_iii,.,i::glJ,-::4ÍtË:.iq-,., _.iÈ*i1:Laf::+,3;{:-*a,Êi..asÈ;::.-:..:!-:::rr:+:,:F2t2different from those obtained with saline or buffer (the reference curve given by theproducer of thrombotest), but which are also far f".; ;;ubl" ;láigor*tio' times of thediluted plasma samples are considcrably longer after three hours incubation at roomtemperature than immediately after tle preparation of the dilutions. rn contrast,with supernatant obtained aftLr centrifugitio'n ,t z0^0óó;ï" B, min. at 4"c (hish-speed supernatant) the cur'e parallels thË producer,u ,uiirr-" o" uïtr;;;";;"; Àd;;-dant of the time of incubatiori of the dilutiàT Htch:-pJrirpJàuruor, therefore, isthe oniy preparation suitable for use m u dilor.ri ir.ïh"-";;";ructions of referencecurves.The differences in coagulation times between low-speed and high_speed supernatantseen in x'ig. 1 are most easily explained ,r "u,o*uJny "-uÀi."1- Ëiïrtorr), still presentin the low-speed supernatant anà adsorbing, ata retuti,r"ry to* ""te, tactors rr, vrr, rx,and X from the dn;tud normal plasma. i"-J""a.1 smail pelret of Ar (oH), can be Íoundat the bottom of the tube af!e1 high-speed centrifugation of the iow-speed supernatant.when reference curves of thrJmboplastins othei than Thrombotest are considerod, ÍÉ;:the influence of al(oH)3 remnants i.'^o"h less obvious. trr-i.ïá"."d partly by t6" ',.."."",lower sensitivitv oi mosl of the other thromboplastins, partly by a factor vrr- orfactor X-like aciivitv often present irr;;;r"y tissue thromboplastins. This might bethe reason why the eïïect has'escap"Jtn"ïu.".oer,s eye for sucli a long time., Sincernany investigators still iefer to percentages obtainec from reference curvesobtained from d'utions of normal prasmà rÀ.itl 1ii(oH), ,a**ào plasma (Leadingffriï;i,|I'), the importance of sraridardizilg 11r" "errt"f,igurion ri"p rt a high g valueThe adverse effects of not recognising al(oH), remnants already haunt the ritera-ture: for instance the Equivarent ï,atio"MetÀoa á"".o.ai"gi9ïïgi. ,ra Denson (r967)for thromboplastin standardization quite recently has undergone a criticism (Litteland Ratnofl 1g6g) which, rooking at ïhe curoes on which the ïriticism is based (page143 and 144 ol this reference), áust be based on the o.ru*r""**u of the afteraetdescribed in this communicatioí. But how many other misunderstandinss do exist ?S,incerely,X). A. Loeliger,L. P. aq,n Halem-Visser.' H. C. HëmkerH aematology B ection anilSectíon ol Bíochemistru I I,Department ol Internal Med,icineUniuersity Hospital,Leid,en, The N etherland,sReferencesBrecs, R" (1972\: Hum:tn Blocd' coaguloti,on, Ha,emostasis a,nil Thrombtsrls. Illackwell scientificPublications, Oxford.Lnloruc Anrrcr,o (1g69):_Con-trol of anticoagulant therapy. Lancot, g, 990,B rccs 'R 'andDnrso r * 'K 'w '8 . ( 1967 ) :  s i anda rd i za t i à í " i t r r " o r " - " r r gep ro th romb in t imo fo r_ the control of articoagulart, therapy. British Medical Journal, -1, g4.Lrrrnr" a' s' and Rarrvorr, o-. o. litiool r A cornparative study of several óhromboplastins used toi"rË."" the one-stage prothrombin time. Thrombosis et diatho"i" nae*o""na!ica suppl. 85,Roceived for publicaíion 14th Decembe r. 197 2.t?{i"',tj:,1í,*,,ï; lffiï";rl:."t:r;1,ï:::-. - Der vertag behàlt sidr aue Reóte, besonders die des Nadrdrud<es,

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Plasma free of factors II, VII, IX, and X:Need for high speed centrifugation after Al(OH)<sub>3</sub> absorption

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