Gilhotra, Ritu

Gupta, Gaurav

Mishra, Anurag

Pathak, Sachchidanand

Rawat, Sarita

Singh, Himmat

Singh, Santosh Kumar

English

Eldorado - Ressourcen aus und für Lehre, Studium und Forschung

EXCLI Journal 2019;18:950-954 – ISSN 1611-2156 
Received: September 26, 2019, accepted: October 10, 2019, published: October 21, 2019 
 
 
950 
Letter to the editor: 
RECENT UPDATES ON DAIDZEIN AGAINST  
OXIDATIVE STRESS AND CANCER 
 
Sarita Rawat, Sachchidanand Pathak, Gaurav Gupta*, Santosh Kumar Singh, Himmat Singh, 
Anurag Mishra, Ritu Gilhotra 
 
School of Pharmacy, Suresh Gyan Vihar University, Mahal Road, Jagatpura, Jaipur, India 
 
* Corresponding author: Gaurav Gupta, School of Pharmacy, Suresh Gyan Vihar  
University, Mahal Road, Jagatpura, Jaipur, India. E-mail: gauravpharma25@gmail.com 
 
 
http://dx.doi.org/10.17179/excli2019-1847 
This is an Open Access article distributed under the terms of the Creative Commons Attribution License  
(http://creativecommons.org/licenses/by/4.0/). 
 
 
Dear Editor, 
Daidzein (7-hydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one) is a naturally occurring 
compound commonly found in soybeans and some other legumes.  Daidzein is an isoflavone 
by nature and isolated from Pueraria Mirifica, having category of biologically active second-
ary metabolites commonly produced in the soybean growth and belong to the group of flavo-
noids. A number of pharmacological activities have been accounted for daidzein, which in-
cludes anti-carcinogenesis, anti-fibrotic, anti-diabetic, cholesterol-lowering and cardiovascu-
lar activity. Daidzein pretreatment was found to diminish the seriousness of mucosal damage 
in a portion subordinate way. Some other therapeutic uses of daidzein are weight reduction, 
decreasing bowels moment, and inflammation associated with histopathological deformities 
(Amaral et al., 2017). Their structure and function are alike to human estrogen, which can 
play a massive role in the prevention of osteoporosis, cancer, and postmenopausal syndromes. 
Uses of daidzein in anticancer activity against ovarian cancer are still limited (Meng et al., 
2017). Epidemiological data suggest that increased utilization of soybean in food results in 
decreased cancer risk. Soybean food is highly recommended in cancer prevention because it 
has a number of anticarcinogens (Yu et al., 2017). The potent antioxidant activity of daidzein 
reported in various in vitro and in vivo studies was conclusively shown in this literature (Ta-
ble 1).  
  
EXCLI Journal 2019;18:950-954 – ISSN 1611-2156 
Received: September 26, 2019, accepted: October 10, 2019, published: October 21, 2019 
 
 
951 
Table 1: Recent updates on daidzein against oxidative stress and cancer 
Key findings Reference 
Daidzein could inhibit 5-FU-induced intestinal mucositis 
through prevention of relevant clinical symptoms, stimula-
tion of the proliferative activity in the epithelium and 
crypts, suppression of oxidative stress and regulation of 
inflammatory homeostasis.  
Atiq et al., 2019 
Soluble CXCL16 and VEGF-A are two promoters of angi-
ogenesis and metastasis in breast cancer. The stimula-
tion of these two angiogenesis-related cytokines might 
represent one of the mechanisms explaining the prolifera-
tive effects of low isoflavone doses in estrogen-dependent 
cells. In estrogen-independent cells, daidzein inhibited 
their secretion, indicating anti-angiogenic properties. 
Uifalean et al., 2018 
The utilization of daidzein is associated with a reduced 
risk of prostate cancer but the direct effects of daidzein on 
the androgen receptor (AR) signaling pathway are not 
well understood.  
Sivoňová et al., 2019 
Cytotoxic effects of daidzein on both MIA PaCa-2 and HT-
29 cell lines occurred in a dose-dependent manner. Daid-
zein exhibits genotoxic potential under in vitro conditions.  
Gundogdu et al., 2018 
Cell models and clinically possible concentrations of soy 
isoflavones do not support the notion that daidzein or oth-
er soy isoflavones can increase the effects of taxane 
chemotherapy in CRPC cell and xenograft models.  
Eskra et al., 2019 
Genistein and daidzein inhibit the accumulation of lipid 
droplets (LDs) by regulating LDs-related factors and lead 
to final apoptosis of colon cancer cells and possible mo-
lecular mechanisms of isoflavones in anti-obesity and an-
ti-tumor functions. 
Liang et al., 2018 
Genistein, daidzein, and ERB-041 decreased ovarian 
cancer cell migration, invasion, proliferation, and bubble 
formation, signifying their roles on ovarian cancer cell me-
tastasis, tumorigenesis, and their potential as other thera-
pies for ovarian cancer patients. 
Chan et al., 2018 
Daidzein exerted neuroprotective effect against ICV‐STZ–
induced behavioral parameters include cognitive deficits 
and locomotor impairment. Where daidzein was able to 
correct oxidative stress in neuronal cells in the brain.  
Wei et al., 2019 
Daidzein enhanced cisplatin-impaired antioxidant defense 
such as reduced glutathione reserve, GPX activity, and 
SOD activity.  
Meng et al., 2017 
Daidzein attenuates CP-induced oxidative stress on blood 
cells and antioxidants in rats. 
Karale and Kamath, 2017 
Daidzein reduces hyperglycemia-induced oxidative stress 
in HUVECs. Thus, daidzein may be a potential therapeu-
tic agent that could reduce hyperglycemia-induced oxida-
tive damage associated with diabetes. 
Park et al., 2016 
Daidzein dosing reduced the B cell population as well as 
IgG1 production, while increased the helper and cytotoxic 
T cell population in females. The shortage of protection or 
exacerbation of T1D can be explained by the reduced B 
cell population and increased regulatory cells, revealing a 
completely unusual mechanism compared to GEN expo-
sure.  
Huang et al., 2019 
 
  
EXCLI Journal 2019;18:950-954 – ISSN 1611-2156 
Received: September 26, 2019, accepted: October 10, 2019, published: October 21, 2019 
 
 
952 
Table 1 (cont.): Recent updates on daidzein against oxidative stress and cancer 
Key findings Reference 
Considering the activity of daidzein as nutraceutical that 
forms complexes with actin that are helpful in the preven-
tion of cancer metastases. 
Budryn et al., 2018 
Anti-proliferative action of 12 anticarcinogens from soy-
bean against MCF-7 and MDA-MB-231 human breast 
cancer cell outline by single or two-way combination 
treatment. There is a synergistic result of the combination 
treatment of genistein plus daidzein MCF-7 cells.  
Zhu et al., 2018 
Daidzein exerts similar activity against SKOV3 cells in 
nude mouse xenograft models and it was shown that dai-
dzein considerably reduced the tumorigenesis in vivo, in-
dicative of the potential for daidzein as a lead molecule in 
the growth of ovarian cancer chemotherapy. 
Hua et al., 2018 
Daidzein may induce apoptosis of choriocarcinoma cells 
in a dose-dependent manner via the mitochondrial apop-
totic pathway. 
Zheng et al., 2018 
Daidzein and equol do not provoke a major up-regulation 
of the transporter expression but instead an inhibition of 
BCRP activity and sensitization to BCRP substrates. 
Rigalli et al., 2019 
E1 and E2 conjugation by low micromolar concentrations 
of daidzein in the human breast cancer cell line MCF7, 
which leads to a small but statistically significant increase 
in unconjugated E2 of approximately 20 %.  
Poschner et al., 2017 
The impact of the dietary isoflavone daidzein on the bio-
chemical fingerprint of two human breast cancer cell lines 
– estrogen-responsive (MCF-7) and estrogen-
unresponsive- was found to induce a noticeable decrease 
in cell growth.  
Medeiros et al., 2016 
Daidzein is just as useful as genistein in protecting cells 
against oxidative damage especially with respect to DNA. 
Antioxidant activity of isoflavones could really contribute 
to the healthy properties of soy. 
Foti et al., 2005 
Oxidative DNA damage by daidzein metabolites plays a 
role in tumor initiation and that cell proliferation by isofla-
vones via ER-ERE binding induces tumor support and/or 
progression, resulting in cancer of estrogen-sensitive or-
gans. 
Murata et al., 2004 
Antioxidant enzyme (AOE) system provoked by daidzein 
affected the oxidant rather than the antioxidant properties 
of daidzein. 
Rohrdanz et al., 2002 
Daidzein supplementation may protect cells from oxida-
tive stress-inducing agents by inhibiting NF-kappa B acti-
vation and decreasing DNA adduct levels. 
Davis et al., 2001 
Daidzein can inhibit choriocarcinoma cell proliferation in 
vitro and in vivo; the underlying mechanism behind the 
inhibitory effects may probably be suppressing ERK 
pathway and afterward arresting cell cycle at G1 phase. 
Zheng et al., 2017 
Biotransformation increased the anti-aromatase activity 
and the anti-tumoral value of daidzein in breast cancer 
cells. Moreover, it clarifies the potential use of daidzein in 
the prevention and/or treatment of ER+ breast cancer. 
Amaral et al., 2017 
 
  
EXCLI Journal 2019;18:950-954 – ISSN 1611-2156 
Received: September 26, 2019, accepted: October 10, 2019, published: October 21, 2019 
 
 
953 
Conflict of interest 
The authors declare no conflict of inter-
est. 
 
REFERENCES 
Amaral C, Toloi MRT, Vasconcelos LD, Fonseca 
MJV, Correia-da-Silva G, Teixeira N. The role of 
soybean extracts and isoflavones in hormone-
dependent breast cancer: Aromatase activity and bio-
logical effects. Food Funct. 2017;8:3064-74. 
Atiq A, Shal B, Naveed M, Khan A, Ali J, Zeeshan S, 
et al. Daidzein ameliorates 5-fluorouracil-induced in-
testinal mucositis by suppressing oxidative stress and 
inflammatory mediators in rodents. Eur J Pharmacol. 
2019;843:292-306. 
Budryn G, Grzelczyk J, Pérez-Sánchez H. Binding of 
red clover isoflavones to actin as a potential mecha-
nism of anti-metastatic activity restricting the migra-
tion of cancer cells. Molecules. 2018;23:E2471. 
Chan KKL, Siu MKY, Jiang YX, Wang JJ, Leung 
THY, Ngan HYS. Estrogen receptor modulators 
genistein, daidzein and erb-041 inhibit cell migration, 
invasion, proliferation and sphere formation via mod-
ulation of fak and pi3k/akt signaling in ovarian can-
cer. Cancer Cell Int. 2018;18:65. 
Davis JN, Kucuk O, Djuric Z, Sarkar FH. Soy isofla-
vone supplementation in healthy men prevents nf-
kappa b activation by tnf-alpha in blood lymphocytes. 
Free Radic Biol Med. 2001;30:1293-302. 
Eskra JN, Schlicht MJ, Bosland MC. Lack of combi-
nation effects of soy isoflavones and taxane chemo-
therapy of castration-resistant prostate cancer. The 
Prostate. 2019;79:223-33. 
Foti P, Erba D, Riso P, Spadafranca A, Criscuoli F, 
Testolin G. Comparison between daidzein and geni-
stein antioxidant activity in primary and cancer lym-
phocytes. Arch Biochem Biophys. 2005;433:421-7. 
Gundogdu G, Dodurga Y, Cetin M, Secme M, Cicek 
B. The cytotoxic and genotoxic effects of daidzein on 
mia paca-2 human pancreatic carcinoma cells and ht-
29 human colon cancer cells. Drug Chem Toxicol. 
2018;1-7;epub ahead of print. 
Hua F, Li CH, Chen XG, Liu XP. Daidzein exerts an-
ticancer activity towards skov3 human ovarian cancer 
cells by inducing apoptosis and cell cycle arrest, and 
inhibiting the raf/mek/erk cascade. Int J Mol Med. 
2018;41:3485-92. 
Huang G, Xu J, Guo TL. Isoflavone daidzein regu-
lates immune responses in the b6c3f1 and non-obese 
diabetic (nod) mice. Int Immunopharmacol. 2019;71: 
277-84. 
Karale S, Kamath JV. Effect of daidzein on cisplatin-
induced hematotoxicity and hepatotoxicity in experi-
mental rats. Indian J Pharmacol. 2017;49:49-54. 
Liang YS, Qi WT, Guo W, Wang CL, Hu ZB, Li AK. 
Genistein and daidzein induce apoptosis of colon can-
cer cells by inhibiting the accumulation of lipid drop-
lets. Food Nutr Res. 2018;62:10.29219/fnr.v62.1384.  
Medeiros PS, Batista de Carvalho AL, Ruano C, 
Otero JC, Marques MP. Raman microspectroscopy 
for probing the impact of a dietary antioxidant on hu-
man breast cancer cells. Food Funct. 2016;7:2800-10. 
Meng H, Fu G, Shen J, Shen K, Xu Z, Wang Y, et al. 
Ameliorative effect of daidzein on cisplatin-induced 
nephrotoxicity in mice via modulation of inflamma-
tion, oxidative stress, and cell death. Oxid Med Cell 
Longev. 2017;2017:3140680. 
Murata M, Midorikawa K, Koh M, Umezawa K, Ka-
wanishi S. Genistein and daidzein induce cell prolif-
eration and their metabolites cause oxidative DNA 
damage in relation to isoflavone-induced cancer of es-
trogen-sensitive organs. Biochemistry. 2004;43:2569-
77. 
Park MH, Ju JW, Kim M, Han JS. The protective ef-
fect of daidzein on high glucose-induced oxidative 
stress in human umbilical vein endothelial cells. Z 
Naturforsch. C. 2016;71(1-2):21-8. 
Poschner S, Maier-Salamon A, Zehl M, Wackerlig J, 
Dobusch D, Pachmann B, et al. The impacts of 
genistein and daidzein on estrogen conjugations in 
human breast cancer cells: A targeted metabolomics 
approach. Front Pharmacol. 2017;8:699. 
Rigalli JP, Scholz PN, Tocchetti GN, Ruiz ML, Weiss 
J. The phytoestrogens daidzein and equol inhibit the 
drug transporter bcrp/abcg2 in breast cancer cells: Po-
tential chemosensitizing effect. Eur J Nutr. 2019;58: 
139-50. 
Rohrdanz E, Ohler S, Tran-Thi QH, Kahl R. The phy-
toestrogen daidzein affects the antioxidant enzyme 
system of rat hepatoma h4iie cells. J Nutr. 2002;132: 
370-5. 
Sivoňová MK, Kaplán P, Tatarková Z, Lichardusová 
L, Dušenka R, Jurečeková J. Androgen receptor and 
soy isoflavones in prostate cancer. Mol Clin Oncol. 
2019;10:191-204. 
EXCLI Journal 2019;18:950-954 – ISSN 1611-2156 
Received: September 26, 2019, accepted: October 10, 2019, published: October 21, 2019 
 
 
954 
Uifalean A, Rath H, Hammer E, Ionescu C, Iuga CA, 
Lalk M. Influence of soy isoflavones in breast cancer 
angiogenesis: A multiplex glass elisa approach. J 
BUON. 2018;23(7):53-9. 
Wei J, Yang F, Gong C, Shi X, Wang G. Protective 
effect of daidzein against streptozotocin-induced alz-
heimer's disease via improving cognitive dysfunction 
and oxidative stress in rat model. J Biochem Mol 
Toxicol. 2019;33:e22319. 
Yu B, Tang DZ, Li SY, Wu Y, Chen M. Daidzein 
promotes proliferation and differentiation in osteo-
blastic oct1 cells via activation of the bmp-2/smads 
pathway. Pharmazie. 2017;72(1):35-40. 
Zheng W, Sun R, Yang L, Zeng X, Xue Y, An R. 
Daidzein inhibits choriocarcinoma proliferation by ar-
resting cell cycle at g1 phase through suppressing erk 
pathway in vitro and in vivo. Oncol Rep. 2017;38: 
2518-24. 
Zheng W, Liu T, Sun R, Yang L, An R, Xue Y. Dai-
dzein induces choriocarcinoma cell apoptosis in a 
dose-dependent manner via the mitochondrial apop-
totic pathway. Mol Med Rep. 2018;17:6093-9. 
Zhu Y, Yao Y, Shi Z, Everaert N, Ren G. Synergistic 
effect of bioactive anticarcinogens from soybean on 
anti-proliferative activity in mda-mb-231 and mcf-7 
human breast cancer cells in vitro. Molecules. 2018; 
23(7): E1557. 
 
 


Recent updates on daidzein against oxidative stress and cancer

https://eldorado.tu-dortmund.de/bitstream/2003/39131/1/Gupta_21102019_proof.pdf

Recent updates on daidzein against oxidative stress and cancer

Abstract

Similar works

Full text

Available Versions

Eldorado - Ressourcen aus und für Lehre, Studium und Forschung