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Differential engulfment of staphylococcus aureus and pseudomonas aeruginosa by monocyte-derived macrophages is associated with altered phagocyte biochemistry and morphology
Authors
JJ Ashworth
A Belboul
+5 more
N Dempsey-Hibbert
CM Liauw
ME Mohtadi
L Pilkington
KA Whitehead
Publication date
30 September 2020
Publisher
EXCLI
Doi
Cite
Abstract
© 2020, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved. Knowledge of changes in macrophages following bacterial engulfment is limited. U937-derived macrophages were incubated with Staphylococcus aureus or Pseudomonas aeruginosa. Morphological and biochemical changes in macrophages following host-pathogen interactions were visualized using Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR) respectively. Principal Component Analysis (PCA) was used to assess the variability in the FTIR spectra. Following host-pathogen interactions, survival of S. aureus was significantly lower than P. aeruginosa (P 99 % of variability in the FTIR spectra explained by the first two principal components. These findings demonstrated that there were clear morphological and biochemical changes in macrophages following engulfment of two different bacterial types suggesting that the biochemical components of the bacterial cell wall influenced the biochemical characteristics and hence the morphology of macrophages in distinct ways
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E-space: Manchester Metropolitan University's Research Repository
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oai:e-space.mmu.ac.uk:626649
Last time updated on 20/10/2020
Edge Hill University Research Information Repository
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oai:pure.atira.dk:publications...
Last time updated on 12/11/2020