The utilization of Electrical Pulse Stimulation (EPS) has been predominantly used to study the physiological, cellular, and molecular responses of excitable cells such as nerve and muscle. Based on previous work on myotubes, non-excitable myoblasts, and non-excitable cancerous rhabdomyosarcoma cells, this study looks into the effects of EPS on breast cancer cells (MCF7s). My aim is to characterize the response of MCF7 cells to EPS and add to a working model of cell cycle arrest, autophagy, and cell death mediated by calcium signaling through cell cycle signaling proteins. This is based on the body of literature detailing the effects of EPS on excitable cell types. Ultimately, by stressing cancer cells with EPS, we can learn more about potential novel modes to induce mechanisms of proliferative failure
