A structured approach to identify biological components most affected by a ship-source oil spill has been developed utilising a suite of criteria to assess vulnerability. Our approach divides criteria into three categories: exposure, sensitivity, and recovery, each encompassing a number of criteria which are envisaged to be consistent and broad enough to be usable in any region in Canada. In support of this, we are working with biologists from other Canadian regions who are currently developing ship-source oil spill response plans (i.e. Pacific, Quebec and Maritimes) to test the usability of this approach in multiple marine environments. For the Pacific region, a full application of this process is underway for the Salish Sea. If successful, we anticipate this approach will be useful for identification of biological components most affected by ship-source oil spills in any marine environment