Beilharz, TH

Formosa, LE

Jackson, TD

Lazarou, M

Muellner-Wong, L

Reljic, B

Ryan, MT

Sharpe, AJ

Stojanovski, D

Stroud, DA

Cell Reports

Mitochondrial complex I harbors 7 mitochondrial and 38 nuclear-encoded subunits. Its biogenesis requires the assembly and integration of distinct intermediate modules, mediated by numerous assembly factors. The mitochondrial complex I intermediate assembly (MCIA) complex, containing assembly factors NDUFAF1, ECSIT, ACAD9, and TMEM126B, is required for building the intermediate ND2-module. The role of the MCIA complex and the involvement of other proteins in the biogenesis of this module is unclear. Cell knockout studies reveal that while each MCIA component is critical for complex I assembly, a hierarchy of stability exists centered on ACAD9. We also identify TMEM186 and COA1 as bona fide components of the MCIA complex with loss of either resulting in MCIA complex defects and reduced complex I assembly. TMEM186 enriches with newly translated ND3, and COA1 enriches with ND2. Our findings provide new functional insights into the essential nature of the MCIA complex in complex I assembly

University of Melbourne Institutional Repository

Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I

Gasse, Stéphanie

Archive Ouverte a LUniversite Lyon 2

Educação não formal : uma contribuição no desenvolvimento territorial integrado

Formosa et al. investigate the function of the MCIA complex in complex I assembly. They demonstrate the requirement of individual components for the formation of complex I intermediates and assembly of the final enzyme. Finally, they characterize the involvement of TMEM186 and COA1 in this process.</p

Formosa, Luke E.

Muellner-Wong, Linden

Reljic, Boris

Sharpe, Alice J.

Jackson, Thomas D.

Beilharz, Traude H.

Stojanovski, Diana

Lazarou, Michael

Stroud, David A.

Ryan, Michael T.

Monash University Research Portal

http://minerva-access.unimelb.edu.au/bitstream/11343/273764/1/PIIS2211124720304411.pdf

Educação não formal : uma contribuição no desenvolvimento territorial integrado

Abstract

Similar works

Full text

Available Versions

University of Melbourne Institutional Repository

Archive Ouverte a LUniversite Lyon 2

Monash University Research Portal