Indonesian Student Association in Thailand (PERMITHA)
Abstract
Polyphenoloxidase (PPO) from Pacific white shrimp was subjected to Pulsed electric field (PEF) at varying specific energy (54-483 kJ/kg) and pulse number (200-600). PPO activity was decreased as both parameters increased (P < 0.05). Among shrimp treated with PEF, those subjected to PEF-T3 (483 kJ/kg, 600 pulses) had the lower melanosis score than other PEF treatments and the control, packaged in polystyrene trays and wrapped with shrink film, during 10 days of storage at 4 °C (P < 0.05). Highest shear force values were noticed with PEF-T3 treated sample at the end of storage period (day 10) (P < 0.05). Microstructural gaping between shrimp muscle fibers was notably higher in PEF-T3. No protein degradation was observed for all samples, regardless of PEF treatments. Lower mesophilic and psychrophilic microbial counts in shrimp were obtained when PEF-T3 was implemented. After 10 days of storage, higher sensory scores of PEF-T3 treated samples were also attained, as compared to others (P < 0.05). Quality deterioration of shrimp was retarded with the aid of PEF
