Castillo Jimenez, Ana

English

DigitalCommons@CSP (Concordia University St. Paul)

++++++++6INTRODUCTIONMETHODSStaphylococcus aureus is a bacteria that is commonly found in the human microbiome. It is an opportunistic pathogen that can cause illness in the host under the right circumstances due to their defining toxins and superantigens. Many strains have developed a resistance to most antibiotics. About 30% of the human population carries S. aureus. CSP has been collecting swabs from various participants in an effort to determine the presence or absence of genes and proteins through the use of PCR and Western blot analysis. Based on the genes and proteins present, the virulence of S. aureus can be examined along with their capabilities of causing disease. To contribute to the previous data collected, twelve samples were analyzed from state fair attendees. A flow chart was created to identify S. aureus in the isolates I was given. Since S. aureus is a Gram positive cocci bacteria that is hemolytic, catalase positive, and produces coagulase, tests were performed to find colonies with matching characteristics. Going forward, there should be an increase in the sample size in order to see a more established pattern.RESULTS● Out of the 12 isolates, 5 were negative for S. aureus and 7 were S. aureus. ● MSA plates were used to test their capabilities to ferment mannitol, which S. aureus is known to do.● Next, the isolates were streaked onto CNA to test their hemolytic properties and isolate gram positive bacteria matching known properties of S. aureus  ● DNase streaking was then done (to test their ability to produce DNase that hydrolyses DNA). S. aureus is DNase positive.● The last round consisted of catalase (to differentiate staphylococci that produce catalase), coagulase (an enzyme produced by S.aureus), and a gram stain (identification of gram positive cocci) in order to confirm the criteria for S. aureus were met. Approximately 26-27% adults carry Staphylococcus aureus in their nasal cavities.Identification of Staphylococcus aureus In State Fair SamplesAna Castillo JimenezFaculty Mentors: Dr. Mandy Brosnahan, Dr. Taylor Mach Science Department, Concordia University, St. PaulStatus of the samples collected for the study thus far.Sample MSA FermentationCNA HemolysisDnase Catalase Coagulase Gram Stain Staph?D2D0058 No NA NA NA NA NA NoD2D0071 Yes Alpha Positive Positive Positive Gram positive cocciYesD2D0096 Yes Alpha Positive Positive Positive Gram positive cocciYesD2D0098 Yes Beta Negative Positive Positive Gram positive cocciNoD2D0099 Yes Alpha Positive Positive Positive Gram positive cocciYesD2D0100 Yes Alpha Positive Positive Positive Gram positive cocciYesD2D0101 No NA NA NA NA NA NoD2D0122 No growth NA NA NA NA NA NoD2D0251 Yes Alpha Positive Positive Positive Gram positive cocciYesD2D0256 Yes Beta Negative Positive Negative Gram positive cocciNoD2D0271 Yes Beta Positive Positive Positive Gram positive cocciYesD2D0278 Yes Beta Positive Positive Positive Gram positive cocciYesREFERENCES AND ACKNOWLEDGEMENTShttps://www.ncbi.nlm.nih.gov/books/NBK8448/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451395/http://textbookofbacteriology.net/staph.htmlhttps://www.merckmanuals.com/home/infections/bacterial-infections-gram-positive-bacteria/staphylococcus-aureus-infections● Dr. B and Dr. Mach for contributing to the verification of results.● The science department for providing us with the tools and resources needed for this research

Identification of Staphylococcus aureus In State Fair Samples

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Identification of Staphylococcus aureus In State Fair Samples

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