Li, Mengyan

English

Digital Commons @ New Jersey Institute of Technology (NJIT)

New Jersey Institute of Technology Digital Commons @ NJIT Chemistry and Environmental Science Syllabi NJIT Syllabi Spring 2020 CHEM 475-102: Biochemistry Lab I (Revised for Remote Learning) Mengyan Li Follow this and additional works at: https://digitalcommons.njit.edu/chem-syllabi Recommended Citation Li, Mengyan, "CHEM 475-102: Biochemistry Lab I (Revised for Remote Learning)" (2020). Chemistry and Environmental Science Syllabi. 160. https://digitalcommons.njit.edu/chem-syllabi/160 This Syllabus is brought to you for free and open access by the NJIT Syllabi at Digital Commons @ NJIT. It has been accepted for inclusion in Chemistry and Environmental Science Syllabi by an authorized administrator of Digital Commons @ NJIT. For more information, please contact digitalcommons@njit.edu.     Chemistry:   Spring 2020 Course Syllabus   NJIT Academic Integrity Code: All Students should be aware that the Department of Chemistry & Environmental Science (CES) takes the University Code on Academic Integrity at NJIT very seriously and enforces it strictly. This means that there must not be any forms of plagiarism, i.e., copying of homework, class projects, or lab assignments, or any form of cheating in quizzes and exams. Under the University Code on Academic Integrity, students are obligated to report any such activities to the Instructor.   COURSE INFORMATION  Course Description: This course will offer the chemistry and related (chemical engineering, biology, bioinformatics, bioengineering) students fundamental laboratory approaches for biochemistry and biotechnology. These experiments will reinforce concepts learned in biochemistry lecture classes.    Number of Credits: 2  Prerequisites: CHEM 244 or CHEM 473 with a grade of C or better.  Course-Section and Instructors  Course-Section Instructor CHEM 475-101 Mengyan Li    Office Hours for All Chemistry & Environmental Science Instructors:  Email: mengyan.li@njit.edu Office Hours: Tue from 1:00-3:00 PM or by appointment  Required Textbook:  Title Fundamental Laboratory Approaches for Biochemistry and Biotechnology Author Alexander J. Ninfa, David P. Ballou and Marilee Benore Edition Second Edition Publisher John Wiley & Sons ISBN # 978-0-470-08766-4  University-wide Withdrawal Date: The last day to withdraw with a W is Monday, April 6, 2020. It will be strictly enforced.  Learning Outcomes: Students can design and perform the research in Biochemistry      POLICIES All CES students must familiarize themselves with, and adhere to, all official university-wide student policies. CES takes these policies very seriously and enforces them strictly.   Grading Policy: The final grade in this course will be determined as follows:  Lab Reports 28% Homework Assignments 16% Midterm Exam 28% Final Exam 28%  Your final letter grade in this course will be based on the following tentative curve:   A 90 - 100 C 70 - 74 B+ 85 - 89 D 60 – 69 B 80 - 84 F 0 – 50 C+ 75 - 79    Attendance Policy: Attendance at classes will be recorded and is mandatory. Each class is a learning experience that cannot be replicated through simply “getting the notes.”  Lab report Policy: Lab report is an expectation of the course and will be used in the determination of the final letter grade as described above. The lab report should be written by your words with the style of the scientific article. All structures of chemicals used in the lab should be included in the method part. Submission due is every Monday 11:59 PM on Canvas (No hard copy submission is accepted).  Homework Policy: Homework assignments are posted on Canvas. Submission due is every Friday 11:59 PM on Canvas (No hard copy submission is accepted).  Exams: There will be one midterm exam and one comprehensive final exam held online during the semester.   Midterm Exam  March 24, 2020 Final Exam April 28, 2020  The final exam will test your knowledge of all the course material taught in the entire course.   Makeup Exam Policy: There will normally be NO MAKE-UP QUIZZES OR EXAMS during the semester. In the event that a student has a legitimate reason for missing a quiz or exam, the student should contact the Dean of Students office and present written verifiable proof of the reason for missing the exam, e.g., a doctor’s note, police report, court notice, etc. clearly stating the date AND time of the mitigating problem. The student must also notify the CES Department Office/Instructor that the exam will be missed so that appropriate steps can be taken to make up the grade.  Cellular Phones: All cellular phones and other electronic devices must be switched off during all class times. Such devices must be stowed in bags during exams or quizzes.   ADDITIONAL RESOURCES  Chemistry Tutoring Center: Located in the Central King Building, Lower Level, Rm. G12. Hours of operation are Monday – Friday 10:00 am - 6:00 pm. For further information please click here.    Accommodation of Disabilities: Office of Accessibility Resources and Services (formerly known as Disability Support Services) offers long term and temporary accommodations for undergraduate, graduate and visiting students at NJIT.  If you are in need of accommodations due to a disability please contact Chantonette Lyles, Associate Director at the Office of Accessibility Resources and Services at 973-596-5417 or via email at lyles@njit.edu. The office is located in Fenster Hall Room 260. A Letter of Accommodation Eligibility from the Office of Accessibility Resources Services office authorizing your accommodations will be required.  For further information regarding self-identification, the submission of medical documentation and additional support services provided please visit the Accessibility Resources and Services (OARS) website at:  http://www5.njit.edu/studentsuccess/disability-support -services/         Course Outline  Lecture Date Topic Assignment 1 01/21 Introduction: Basic practices and techniques in the biochemistry laboratory   2 01/28 Spectrophotometry: Create a standard curve and determine concentration of unknown using spectrophotometer  Lab report 3 02/04 Quantification of protein concentration: Determine the concentration of a protein using the Bradford assay  Lab report 4 02/11 Chromatography: Separate a mixture of biomolecules based on size using gel filtration chromatography  Lab report 5 02/18 Gel electrophoresis of proteins: Separate a mixture of proteins using gel electrophoresis and determine the size Lab report 6 02/25 Protein isolation: Purify a single protein from a complex mixture of proteins  Lab report 7 03/03 SDS-PAGE: Determine the purity of the isolated protein Lab report 8 03/10 Enzyme kinetics: Determine the kinetic parameters (Kcat and KM) of an enzyme  Lab report 9 03/24 Midterm Exam  10 03/31 Polymerase chain reaction technology: Amplify DNA using PCR  Homework 11 04/07 Miniprep: Isolate and characterize a plasmid.  Homework 12 04/14 Transformation: Insert DNA into E. coli and select positive cells                Homework 13 04/21 Bioinformatics: Use the internet to search databases and visualize molecular structures  Homework 14 04/28 Final Exam        Updated by March 2020 Department of Chemistry & Environmental Sciences  Course Syllabus, Spring 2020 

CHEM 475-102: Biochemistry Lab I (Revised for Remote Learning)

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CHEM 475-102: Biochemistry Lab I (Revised for Remote Learning)

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