Attempts were made to clone the yeast adenosine-5'-monophosphate deaminase (AMP deaminase) gene from S. cerevisiae using a number of strategies. Initially, it was hoped that overproduction of AMP deaminase could be detected in yeast, by selecting for resistance to the AMP deaminase inhibitor, deoxycoformycin (dCF). This involved the construction of ade, hpt1 yeast strains in which the AMP deaminase reaction is the major source of inosine-5'-monophosphate (IMP). Although yeast AMP deaminase is sensitive to dCF in vitro no effect was observed in vivo (possibly due to lack of uptake of the drug)
