A series of low molecular weight thermoreversible cystine hydrogelators were
synthesised via solid-phase chemistry. Novel hydrogels were found to gelate at
concentrations of <2 mM using microwave super-heating. Benzoyl cystine amide
derivative hydrogel, which could form at a concentration of 0.5 mM, equivalent to
0.022% w/w of gelator with respect to water (an incredible 111,000 molecules of
water gelated per single molecule of gelator), was applied to cell culture of cervical
cancer (HeLa) cells, which were found to distribute within the gel. Hydrogels were
produced on a microarray format using a novel strategy involving deposition of
hydrogel solutions by inkjet printing. The incorporation of fluorescent dye
(Rhodamine B) into hydrogels provided a novel means for studying hydrogel
morphology.Reversible boronate chemistry was implemented for the capture and release of
proteins and peptides onto a solid-support as part of a modified peptide enrichment
strategy. The strategy was proven following synthesis of hydroxamic acid and
catechol modified peptides and a study of their interaction with solid-supported
phenylboronic acid. NHS active ester affinity tags and cross-linkers were synthesised
and applied to a 3D proteomics cross-linking analysis pipe-line. The introduction of a
PEG unit led to a cross-linker with increased hydrophilicity and improved
observation of both inter and intra-protein cross-links by mass spectrometry
