Chaturvedi, Tanmay

Skovhus, Torben

Thomsen, Mette Hedegaard

English

    Aalborg UniversitetInvestigation of natural antimicrobial compounds for prevention of microbiologicallyinfluenced corrosion (MIC)Chaturvedi, Tanmay; Skovhus, Torben; Thomsen, Mette HedegaardPublication date:2019Link to publication from Aalborg UniversityCitation for published version (APA):Chaturvedi, T., Skovhus, T., & Thomsen, M. H. (2019). Investigation of natural antimicrobial compounds forprevention of microbiologically influenced corrosion (MIC). Poster presented at 7th International Symposium onApplied Microbiology and Molecular Biology in Oil Systems , Halifax, Canada.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            ? You may not further distribute the material or use it for any profit-making activity or commercial gain            ? You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us at vbn@aub.aau.dk providing details, and we will remove access tothe work immediately and investigate your claim.Downloaded from vbn.aau.dk on: November 24, 2020RESULTSInvestigation of natural antimicrobial compounds for prevention of microbiologically influenced corrosion (MIC)Tanmay Chaturvedi 1*, Torben L Skovhus2, Mette H Thomsen 11) Aalborg University, Department of Energy Technology, Esbjerg, Denmark2) VIA University College, Horsens, Denmark*Corresponding author, e-mail: tac@et.aau.dk , Phone: +45 7144167423 59 > 1< 110  0 1 10 100CS SS CS SS CS SSControl Continuous PulseqPCR (copies/µL) in Millions qPCR (Bacteria) of biofilm microorganisms:EXPERIMENTAL SETUPCurrent options and the necessity to apply natural antimicrobial compounds • Eliminating sulfates through filtration of seawater is a possible way to control sulfides - however it is expensive.• The addition of nitrate to injection water could abate H2S production - but it requires repeated treatments and is associated with high chemical costs. Addition of nitratemight also increase the biomass of microorganisms in the water, causing plugging and even increased corrosion rates in some fields.• The current industrial methods of controlling MIC are maintenance pigging and chemical treatment (biocides and corrosion inhibitors).• A combination of mechanical cleaning and chemical treatment are the most efficient techniques currently. Chemical controls are generally considered to be the mosteffective in performance and cost. While biocides readily destroy planktonic cells, biofilm cells located on the pipeline surfaces are protected by a polysaccharidescovering and ward off the effects of toxic biocides.0306090120150180CS SS CS SS CS SSControl Continous PulsecATPx 106(ME/cm2)ATP measurement of biofilm collected on coupon surface:4 times reduction in biofilms!3 times reduction in biofilms!• 3 reactors (Control, Continuous and Pulse) inoculated with sediments from Wadden Sea.• Nitrogen was flushed to maintain anaerobic conditions.• Each reactor has 8 rods, and 3 coupons in each rod, allowing for 24 coupon positions. Out of the 24available positions, 4 stainless steel (SS) and 4 carbon steel (CS) coupons were used in EACH reactor.• Extractives mixture is added to Continuous and Pulse reactors. The extractives mixture is obtained fromhalophytes (Patent pending)• 10% (v/v) extract mix is added to the Continuous reactor• 3 pulses, of 5, 10, and 15 % (v/v) are added on Day 14, 20, and 26 respectively to the Pulse reactor• Coupons are rinsed and sent for analysis at the end of the experiments.• Significant inhibition of MIC bacteria was observed in liquid samples from the reactors treated with Halophytic Plant Extracts.• Biofilm formation was reduced by three times on carbon steel and four times on stainless steel coupons in reactors treated with Halophytic Plant Extracts.• Visible and measurable reduction in pitting and corrosion damages on carbon steel coupons was observed with the help of 3D surface scanning (data not shown).• Addition of Halophytic Plant Extracts reduce the microbiological abundance (number of microorganisms measured by qPCR) by 20 times for Carbon steel and by at least 5 times for Stainless steel. • Halophytic Plant Extracts DO reduce the microbiological diversity, which was measured for top 40 genera (diversity index, data not shown).• Characterization of halophytic plant extracts and formulations.• Compare pulse versus continued flow, variation in concentrations, contact time, and  frequency of extractive addition. • Techno-economic evaluation of the natural biocide production.ACKNOWLEDGEMENT: We thank Danish Hydrocarbon Research and Technology Centre (DHRTC) for funding this project.MAJOR FINDINGSWHAT’S NEXTBACKGROUND AND NEEDSHeat-map of key genera:PCA plot of all samples:

Investigation of natural antimicrobial compounds for prevention of microbiologically influenced corrosion (MIC)

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Investigation of natural antimicrobial compounds for prevention of microbiologically influenced corrosion (MIC)

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