Nielsen, Boye S

Nielsen, Søren

Pedersen, Sine H

Thomsen, Christian

Vyberg, Mogens

English

Immunohistochemical (IHC) quantification of estrogen receptor-α (ER) is used for assessment of treatment regimen in breast cancer. Different ER IHC assays may produce diverging results, because of different antibody clones, protocols, and stainer platforms. Objective tissue-based techniques to assess sensitivity and specificity of IHC assays are therefore needed. We tested the usability of ER mRNA-in situ hybridization (mRNA-ISH) in comparison with assays based on clones SP1 and 6F11. We selected 56 archival specimens according to their reported ER IHC positivity, representing a wide spectrum from negative to strongly positive cases. The specimens were used to prepare 4 TMAs with 112 cores. Serial sections of each TMA were stained for ER and pan-cytokeratin (PCK) by IHC and ESR1 (ER gene) by mRNA-ISH. Digital image analysis (DIA) was used to determine ER IHC H-score. ESR1 mRNA-ISH was scored both manually and by DIA. DIA showed a nonlinear correlation between IHC and ESR1 mRNA-ISH with R 2 -values of 0.80 and 0.78 for the ER antibody clones SP1 and 6F11, respectively. Comparison of manual mRNA-ISH scoring categories and SP1 and 6F11 IHC H-scores showed a highly significant relationship (P&lt;0.001). In conclusion, the study showed good correlation between mRNA-ISH and IHC, suggesting that mRNA-ISH can be a valuable tool in the assessment of the sensitivity and specificity of ER IHC assays. </p

VBN (Videnbasen)  Aalborg Universitets forskningsportal

Estrogen Receptor-α Quantification in Breast Cancer:Concordance Between Immunohistochemical Assays and mRNA-In Situ Hybridization for ESR1 Gene

Christian Thomsen

Søren Nielsen

Boye S. Nielsen

Sine H. Pedersen

Mogens Vyberg

Crossref

Estrogen Receptor-α Quantification in Breast Cancer: Concordance Between Immunohistochemical Assays and mRNA-In Situ Hybridization for ESR1 Gene

https://vbn.aau.dk/ws/files/343444551/Thomsen_et_al._2020_Estrogen_Receptor_Quantification_in_Breast_Cancer.pdf

Estrogen Receptor-α Quantification in Breast Cancer:Concordance Between Immunohistochemical Assays and mRNA-In Situ Hybridization for ESR1 Gene

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VBN (Videnbasen) Aalborg Universitets forskningsportal

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