Aamand, Jens

Ellegaard-Jensen, Lea

Hestbjerg Hansen, Lars

Hylling, Ole

Madsen, Henrik Tækker

Muff, Jens

Nikbakht Fini, Mahdi

English

    Aalborg UniversitetRetentate from Reverse Osmosis Stimulates Degradation Potential of BioaugmentationCandidate Aminobacter sp. MSH1Hylling, Ole; Nikbakht Fini, Mahdi ; Muff, Jens; Madsen, Henrik Tækker; Aamand, Jens;Hestbjerg Hansen, Lars; Ellegaard-Jensen, LeaPublication date:2018Document VersionPublisher's PDF, also known as Version of recordLink to publication from Aalborg UniversityCitation for published version (APA):Hylling, O., Nikbakht Fini, M., Muff, J., Madsen, H. T., Aamand, J., Hestbjerg Hansen, L., & Ellegaard-Jensen, L.(2018). Retentate from Reverse Osmosis Stimulates Degradation Potential of Bioaugmentation CandidateAminobacter sp. MSH1. Poster presented at 17th International Symposium on Microbial Ecology, Leipzig,Germany.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            ? You may not further distribute the material or use it for any profit-making activity or commercial gain            ? You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us at vbn@aub.aau.dk providing details, and we will remove access tothe work immediately and investigate your claim.Downloaded from vbn.aau.dk on: December 27, 20201. Ellegaard-Jensen, L., et al., Groundwater contamination with 2,6-dichlorobenzamide (BAM) and perspectives for its microbial removal. Appl Microbiol Biotechnol, 2017. 101(13): p. 5235-5245.2. Albers, C.N., et al., Degradation of trace concentrations of the persistent groundwater pollutant 2,6-dichlorobenzamide (BAM) in bioaugmented rapid sand filters. Water Res, 2015. 83: p. 61-70.Retentate from Reverse Osmosis Stimulates Degradation Potential of Bioaugmentation Candidate Aminobacter sp. MSH1Ole Hyllinga, Mahdi Nikbakht Finib, Jens Muffb, Henrik Tækker Madsenc, Jens Aamandd, Lars H. Hansena, Lea Ellegaard-JensenaaSection for Environmental Microbiology & Biotechnology, Dept. Environmental Science, Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark.bSection of Chemical Engineering, Dept. of Chemistry and Bioscience, Aalborg University, Niels Bohrs vej 8, 6700 EsbjergcSection for Sustainable Biotechnology, Dept. of Chemistry and Bioscience, Aalborg University, A.C. Meyers Vænge 15, 2450 Copenhagen SVdDept. Geochemistry, Geological Survey of Denmark & Greenland, Ø. Voldgade 10, 1350, Copenhagen, Denmark.           BACKGROUND & SUMMARY Groundwater is an important resource in drinking water production, but is threatened by recalcitrant pollutants, such as the pesticide residue 2,6-dichlorobenzamide (BAM)1. We aim to combine retentate from membrane separation and bioaugmentation as a novel biotechnology for removal of micropollutants (BOX 1). Here we show results (BOX 3) from batch experiment(s) , using a BAM-degrading bacteria, Aminobacter sp. MSH1, in untreated and retentate water (BOX 2).SCIENTIFIC QUESTIONS How is BAM degradation potential of Aminobacter sp. MSH1 affected in different membrane retentates versus untreated water?  Is there an optimal retentate with respect to BAM degradation? Is MSH1 population negatively affected in the retentates?BOX 1 – CONCEPT & HYPOTHESISOne of the main challenges in sand filter bioaugmentation is low pollutant concentrations as this can result in loss of degrader population and/or activity over time, due to starvation2.Membrane separation will increase concentration of target pollutant in retentate, which in turn could act as feed for a sand filter, dedicated to bioaugmentation.Hypothesis: increased pollutant concentration should stimulate activity and enhance overall fitness of degrader bacteria.(www.mem2bio.dk)CONCLUSIONS MSH1 degradation potential is stimulated in the membrane retentates Stimulation of degradation potential in retentates is irrespective of BAM concentation MSH1 population remains stableREVERSE OSMOSISUNTREATED RETENTATESCollected waterMSH1 14C BAM+ +COMMUNITYMICROCOSMS (10 ⁰C)Aminobacter sp. MSH1:DEGRADATION POTENTIAL• Liquid scintillation countingENUMERATION• qPCR of degrader gene bbdA50%90%80%(RETENTION LEVELS)HARDSOFTBOX 2 - EXPERIMENTAL SETUP & WORKFLOWdHPERSPECTIVES Retentate from membrane separation could act as stimulating feed for  bioaugmented sand filters in drinking water production – prolonging the stability of introduced degrader strain Giving way for a novel environmentally friendly biotechnology for eliminating  micropollutants during water purificationohy@envs.au.dk90% Retentateuntreated 50% Retentate 80% Retentate% 14C REMOVAL % 14C MINERALIZEDSame 14C-BAM CONC.MSH1 POPULATIONt2Different14C-BAM CONC.Different14C-BAM CONC.Same 14C-BAM CONC.BOX 3 - RESULTS50% Retentate80% Retentate90% RetentateControl (untreated)Control (90% retentate)Untreated90% RetentateControl (untreated) Control (90% retentate) UntreatedNON-INOCULATED INOCULATED w. MSH1NON-INOCULATED INOCULATED w. MSH1

Retentate from Reverse Osmosis Stimulates Degradation Potential of Bioaugmentation Candidate <i>Aminobacter</i> sp. MSH1

https://vbn.aau.dk/ws/files/296238372/ISME17_POSTER_OH.pdf

Retentate from Reverse Osmosis Stimulates Degradation Potential of Bioaugmentation Candidate <i>Aminobacter</i> sp. MSH1

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