Erban, Alexander

Fischer, Axel

Giavalisco, Patrick

K Hincha, Dirk

Kopka, Joachim

Mubeen, Umarah

Pagter, Majken

Schaarschmidt, Stephanie

Sprenger, Heike

Walther, Dirk

Zuther, Ellen

English

Plants from temperate regions can be cold acclimated by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold acclimation has been investigated in detail, little is known about the molecular regulation of deacclimation of cold acclimated plants under warm conditions. Transcript profiling identified genes encoding transcription factors that were transiently induced during the first 24 h of deacclimation, constituting potential regulators determining the rate of loss of freezing tolerance under these conditions. We are currently characterizing the mode of action of the first candidates. In addition, after cold acclimation at 4°C and a deacclimation phase at 20°C, a second treatment at 4°C improved the freezing tolerance of Arabidopsis compared to plants that had only received one cold treatment. This indicates that the plants remembered the first cold treatment and reacted more efficiently to the second cold acclimation. To identify transcripts, lipids and metabolites related to this cold memory, we used Illumina-based RNA-Seq, LC-MS-based lipidomics and GC-MS-based metabolite profiling. We are currently analyzing candidate genes with the aim to identify regulators of cold memory in Arabidopsis. Plants from temperate regions can be cold acclimated by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold acclimation has been investigated in detail, little is known about the molecular regulation of deacclimation of cold acclimated plants under warm conditions. Transcript profiling identified genes encoding transcription factors that were transiently induced during the first 24 h of deacclimation, constituting potential regulators determining the rate of loss of freezing tolerance under these conditions. We are currently characterizing the mode of action of the first candidates. In addition, after cold acclimation at 4°C and a deacclimation phase at 20°C, a second treatment at 4°C improved the freezing tolerance of Arabidopsis compared to plants that had only received one cold treatment. This indicates that the plants remembered the first cold treatment and reacted more efficiently to the second cold acclimation. To identify transcripts, lipids and metabolites related to this cold memory, we used Illumina-based RNA-Seq, LC-MS-based lipidomics and GC-MS-based metabolite profiling. We are currently analyzing candidate genes with the aim to identify regulators of cold memory in Arabidopsis. <br/

    Aalborg UniversitetCold acclimation, deacclimation and memory in ArabidopsisK Hincha, Dirk; Schaarschmidt, Stephanie; Fischer, Axel; Erban, Alexander; Pagter, Majken;Mubeen, Umarah ; Walther, Dirk ; Giavalisco, Patrick ; Kopka, Joachim ; Sprenger, Heike;Zuther, EllenPublication date:2018Link to publication from Aalborg UniversityCitation for published version (APA):K Hincha, D., Schaarschmidt, S., Fischer, A., Erban, A., Pagter, M., Mubeen, U., Walther, D., Giavalisco, P.,Kopka, J., Sprenger, H., & Zuther, E. (2018). Cold acclimation, deacclimation and memory in Arabidopsis.Abstract from SEB Florence 2018, Italy.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            ? You may not further distribute the material or use it for any profit-making activity or commercial gain            ? You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us at vbn@aub.aau.dk providing details, and we will remove access tothe work immediately and investigate your claim.Downloaded from vbn.aau.dk on: December 27, 2020Cold acclimation, deacclimation and memory in Arabidopsis  Dirk K. Hincha1, Stephanie Schaarschmidt1, Axel Fischer1, Alexander Erban1, Majken Pagter2, Umarah Mubeen1, Dirk Walther1, Patrick Giavalisco1,3, Joachim Kopka1, Heike Sprenger1,4, Ellen Zuther1 1Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany 2 Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark  3Max-Planck-Institut für Biologie des Alterns, Joseph-Stelzmann-Str. 9b, 50931 Köln, Germany 4VIB-UGent Center for Plant Systems Biology, Ghent University, Technologiepark 927, 9052 Ghent, Belgium  Plants from temperate regions can be cold acclimated by exposure to low, but non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold acclimation has been investigated in detail, little is known about the molecular regulation of deacclimation of cold acclimated plants under warm conditions. Transcript profiling identified genes encoding transcription factors that were transiently induced during the first 24 h of deacclimation, constituting potential regulators determining the rate of loss of freezing tolerance under these conditions. We are currently characterizing the mode of action of the first candidates. In addition, after cold acclimation at 4°C and a deacclimation phase at 20°C, a second treatment at 4°C improved the freezing tolerance of Arabidopsis compared to plants that had only received one cold treatment. This indicates that the plants remembered the first cold treatment and reacted more efficiently to the second cold acclimation. To identify transcripts, lipids and metabolites related to this cold memory, we used Illumina-based RNA-Seq, LC-MS-based lipidomics and GC-MS-based metabolite profiling. We are currently analyzing candidate genes with the aim to identify regulators of cold memory in Arabidopsis.    

Cold acclimation, deacclimation and memory in Arabidopsis

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Cold acclimation, deacclimation and memory in Arabidopsis

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