Albertsen, Mads

Christensen, Morten Lykkegaard

Hansen, Susan Hove

Jørgensen, Mads Koustrup

Larsen, Poul

Nielsen, Per Halkjær

Nierychlo, Marta

English

The activated sludge process is key in modern wastewater treatment. The microbial community composition must be stable in order to maintain the desired nutrient removal. In addition, bacterial morphology, mode of growth and extracellular polymeric substances composition determine floc size, shape and strength, which in turn influence important sludge properties. Optimal sludge properties are important for the wastewater treatment plant operation and eventually effluent quality. The aim of this study was to established the link between the microbial community structure and physico-chemical sludge characteristics and thereby provide a better understanding of the activated sludge process.DNA-based identification of microorganisms using 16S rRNA gene amplicon sequencing has been proved as a reliable and reproducible method for identification and quantification studies of microbial communities in activated sludge and for the demonstration of their significance for plant operation and stability. 16S rRNA gene amplicon sequencing has been developed over the past few years and is now ready to use for more comprehensive studies related to plant operation and optimization thanks to short analysis time, low cost, high throughput, and high taxonomic resolution.In this study, we found that the composition of microbial community differs between different sludge fractions and that specific bacteria are enriched in defloculated fraction (supernatant) and in the fraction loosely bound to the floc. In addition, individual bacterial types were found to correlate with the defined floc properties.<br/

    Aalborg UniversitetInfluence of microbial community composition on activated sludge floc propertiesHansen, Susan Hove; Nierychlo, Marta; Larsen, Poul; Jørgensen, Mads Koustrup; Albertsen,Mads; Christensen, Morten Lykkegaard; Nielsen, Per HalkjærPublication date:2018Link to publication from Aalborg UniversityCitation for published version (APA):Hansen, S. H., Nierychlo, M., Larsen, P., Jørgensen, M. K., Albertsen, M., Christensen, M. L., & Nielsen, P. H.(2018). Influence of microbial community composition on activated sludge floc properties. Poster presented at17th International Symposium on Microbial Ecology, Leipzig, Germany.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            ? You may not further distribute the material or use it for any profit-making activity or commercial gain            ? You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us at vbn@aub.aau.dk providing details, and we will remove access tothe work immediately and investigate your claim.Downloaded from vbn.aau.dk on: December 27, 2020The activated sludge process is key in modern wastewater treatment. The activated sludge ecosystem contains a core community of abundant organisms and it must be stable in order to maintain the desired nutrient removal. In addition, bacterial morphology, mode of growth and extracellular polymeric substances (EPS) composition determine floc size, shape and strength, which in turn influence sludge properties that are important for plant operation. A few organisms have been correlated with different sludge properties, however, the picture is far from complete.• To investigate whether the microbial community composition differs between the flocs and the supernatant (bulk water) and whether certain bacterial species correlate to floc strength.Influence of microbial community composition on activated sludge floc propertiesAAUshh@bio.aau.dk              @SusanHoveSusan H. Hansen, Marta Nierychlo, Poul Larsen, Mads K. Jørgensen, Mads Albertsen, Morten L. Christensen, Per H. NielsenCenter for Microbial Communities, Department Of Chemistry And Bioscience, Aalborg University, Denmark• Microbial community composition relates to different activated sludge fractions.• Acrobacter and Sulfuritalea are enriched in the bulk water fraction and in the fraction loosely bound to the floc and could therefore be associated as poor floc formers.• Dechloromonas and Tetrasphaera abundances decreased in supernatants and could therefore be associated with potential strong floc formers.• Sulfuritalea and Tetrasphaera can be correlated to sludge characteristics that are important for the proper plant operation (SVI and shear sensitivity).B a c k g r o u n dM e t h o d sC o n c l u s i o n sR e s u l t sSludge fractions16S amplicon sequencingShear600 rpm, 2 hoursActivated sludge from 23 WWTPsCentrifugationSupernatant after shear– loosely bound fractionCentrifugationSupernatant – bulk waterTotal sludgeCommunity composition relates to different activated sludge fractions from 23 WWTPsMultivariate analysis visualized the relationship between all samples analyzed from 23 WWTPs.Samples grouping into individual sludge fractions (total sludge – bulk water –loosely bound fraction) can be  clearly observed. WWTP city names are presented.Comparison of relative abundance between the different activated sludge fractions Enrichment of bacteria in different activated sludge fractions Bacteria enriched in the supernatant compared to total sludge. Highlighted bacteria seem to contribute to poor floc properties.EnrichmentBacteria enriched in the supernatant after shear compared to supernatant. Highlighted bacteria seem to be loosely attracted to the sludge flocs.EnrichmentCorrelation between sludge parameters and bacteria in the sludge fractionsSpearman correlation of bacteria present in different sludge fractions with important sludge characteristics: Sludge Volume Index (SVI) and shear sensitivity. Bacteria that were strongly correlated (-0.6>R>0.6) with the mentioned parameters are listed above.One abundant OTU, belonging to the class of Betaproteobacteria, seems to have a positive impact on SVI.High abundance of Sulfuritalea correlates with high shear sensitivity, whereas the presence of Tetrasphaera correlates to low shear sensitivity.AimActivated sludge was collected from 23 wastewater treatment plants (WWTP) and physio-chemical sludge characteristics were determined. Each sludge sample was then split in three fractions.SLUDGE VOLUME INDEXTotal sludge Supernatant after shearOTU taxonomic classification abundance [%] R OTU taxonomic classification abundance [%] R276 g__Lactococcus 0.1 0.8 11683 f__Caldilineaceae 0.9 0.69794 g__Arcobacter 0.2 0.8 11886 g__Caldilinea 0.2 0.711683 f__Caldilineaceae 3.2 0.7 12307 g__Pseudorhodobacter 0.2 0.711886 g__Caldilinea 0.1 0.7 4117 g__Gordonia 0.2 0.912307 g__Pseudorhodobacter 0.3 0.7 8679 g__Fodinibacter 0.2 0.94117 g__Gordonia 0.2 0.7 5480 g__Nocardioides 0.1 0.78679 g__Fodinibacter 0.3 0.7 10334 g__Thermomonas 0.1 0.713221 g__Streptococcus 0.1 0.7 8804 g__Alicycliphilus 4.6 0.85480 g__Nocardioides 0.1 0.6 13375 g__Parabacteroides 0.2 0.65643 g__Ferruginibacter 0.2 0.6 975 g__Mycobacterium 0.3 0.67904 f__Chitinophagaceae 0.3 0.6 15219 g__Brevundimonas 0.1 0.66268 g__Leucobacter 0.2 0.9 2634 g__Enterococcus 0.1 0.749 g__BS063 0.3 0.7 13221 g__Streptococcus 0.3 0.75387 g__Geothrix 0.4 -0.8 6930 g__HF_BF39 0.4 0.713371 g__Nannocystis 0.2 -0.7 2741 g__B1-K2-141 0.5 0.61858 g__A0837 0.3 -0.7 2681 g__MK04 0.2 0.614179 g__Sulfuritalea 0.4 -0.7 5384 f__Rhodobacteraceae 0.3 0.76532 g__Ferruginibacter 0.4 -0.7 954 f__Phyllobacteriaceae 0.3 0.69778 g__Sulfuritalea 0.5 -0.7 7086 f__Lachnospiraceae 0.1 0.813898 g__CandidatusEpiflobacter 0.7 -0.6 4378 f__Lachnospiraceae 0.1 0.615957 f__Caulobacteraceae 0.1 -0.8 5387 g__Geothrix 0.1 -0.85957 f__AKYH767 0.1 -0.7 12821 c__Betaproteobacteria 7.6 -0.86267 o__Rhizobiales 0.3 -0.7 196 f__env.OPS_17 0.3 -0.610601 f__0319-6G20 0.1 -0.7SHEAR SENSITIVITYTotal sludge Supernatant after shearOTU taxonomic classification abundance [%] R OTU taxonomic classification abundance [%] R5954 g__Trichococcus 2.1 0.6 14179 g__Sulfuritalea 3.2 0.712837 g__Pseudacidovorax 0.3 0.7 7201 g__Bacteriovorax 0.1 0.610410 g__Proteiniclasticum 0.2 0.7 6275 g__Tetrasphaera 1.5 -0.8831 g__Thauera 0.2 0.8 13677 p__WCHB1-60 0.2 -0.714300 f__Propionibacteriaceae 0.1 -0.6 6532 g__Ferruginibacter 0.2 -0.7194 g__Hyphomicrobium 0.9 -0.6 11480 g__rJ14 0.2 -0.710225 f__Kineosporiaceae 0.2 -0.62101 g__QEDR3BF09 0.3 -0.76532 g__Ferruginibacter 0.4 -0.6 negative influence on parameter13898 g__CandidatusEpiflobacter 0.7 -0.6 positive influence on parameter3086 g__Tessaracoccus 0.1 -0.716S rRNA gene amplicon sequencingBioinformaticsDNAExtractionSample preparationTotal sludgeSupernatantSupernatantafter shearMiDAStaxonomy+Relative abundance of 10 most frequently occurring genera compared between the different sludge fractions .The variation between the WWTPs is captured by the boxplot width.Acrobacter seems to be linked to the loosely bound sludge fraction, whereas especially Dechloromonasand Tetrasphaera could be potential strong floc formers.

Influence of microbial community composition on activated sludge floc properties

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Influence of microbial community composition on activated sludge floc properties

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