When damage occurs, there are changes in the structural stiffness. This causes changes in the vibrational information, such as translation and rotation angle of the joints in the structure. This paper presents a sensitivity study of translation and slope of the first-order mode shape of the joints in the frame structure, and which will contribute to structural damage identification further. Starting from the equation of natural vibration of frame structure, and according to the characteristics of the stiffness matrix, derive sensitivity coefficient expression of translation and slope of first-order mode shape of the joints in the structure, and obtain the first-order relative sensitivity coefficient of the first story is always smaller than zero when single column of the first story damage occurs. The numerical analysis of the four stories three spans frame shows consistent results with the formula derivation