Dynamic response of assembled structures is strongly dependent on the mechanical behavior of joint interfaces. However, the effect of mechanical joints on structural dynamics is not yet fully understood due to the complex multi-scale, multi-physics and nonlinear characteristics of frictional contact interfaces. A monolithic beam without joint interfaces and a jointed beam with a typical shear lap joint are fabricated. Both structures are subjected to hammer impact excitation to identify the nonlinear effect of joint interfaces. The time-domain responses of both structures are first compared directly to identify the effect of joints. Experimental modal analysis is further used to yield mode shape, frequency and modal damping. The effects of joint interfaces on structural modal properties are investigated. Finally, the wavelet transform analysis and empirical mode decomposition are used to analyze recorded time-domain signals to quantify the effect of joint interfaces on dynamic behavior in time-frequency domain. The results show that joint interfaces play a critical role in dynamic response of assembled structure. The effective stiffness and damping of the lap joint bolt are amplitude dependent. Nonlinear effect of joints on dynamical response is obvious in high-frequency