1. Morphine, codeine and heroin in amounts of 0.5 to 1.0 mg.
were adsorbed quantitatively from pure water -alcohol (1 :4 by volume) solutions containing from 5 to 20 per cent. trichloracetic
acid and from 1 to 4 per cent. sodium chloride. The adsorption
was made on an acid column (pH 6.5 to 7.0) from acid solutions
(pH 6.5).
2. The three alkaloids were adsorbed quantitatively from
tissue extracts, blood filtrates and urine filtrates only on alkaline
columns (pH 7.5 -3.0) from alkaline solutions (pH 8.0 -8.5).
3. Mixtures of morphine and heroin in pure solutions were
separated into the individual components by selective adsorption
of the heroin on methyl alcohol treated columns.
4. Separation of mixtures of morphine and codeine in pure
solution was also accomplished by selective adsorption of the codeine on methyl alcohol treated columns.
5. The separation into the individual components of mixtures
of codeine and heroin has not been successful under the conditions
described.
6. Evidence for the mutual interference of alkaloids on adsorption
from acid tissue extracts or silicate -containing solution
on acid columns was given.
7. The adsorption method was extended for the separation of
barbiturates from the alkaloids.
8. Barbiturates were adsorbed quantitatively in amounts from
1.0 to 5.0 mg. on activated charcoal from pure water- alcohol trichloracetic
acid solutions either at pH 5.5 or pH 8.5.
9. Barbiturates were adsorbed quantitatively from tissue extracts,
blood and urine filtrates only in the acid range (pH 5.5)
