In many different insect species the highly conserved neuropil regions known as the central
complex or central body complex have been shown to be important in behaviours such as
locomotion, visual memory and courtship conditioning. The aim of this project is to generate accurate quantitative neuroanatomy of the central complex in the fruit fly Drosophila melanogaster.
Much of the authoritative neuroanatomy of the fruit fly from past literature has been derived
using Golgi stains, and in important cases these data are available only from 2D camera lucida
drawings of the neurons and linguistic descriptions of connectivity. These cannot easily be
mapped onto 3D template brains or compared directly to our own data. Using GAL4 driver
and reporter constructs, some of the findings within these studies could be visualized using
immunohistochemistry and confocal microscopy. A range of GAL4 driver lines were selected that
particularly had prominent expression in the fan-shaped body. Images of brains from these lines
were archived using a web-based 3D image stack archive developed for the sharing and backup
of large confocal stacks. This is also the platform which we use to publish the data, so that
other researchers can reuse this catalogue and compare their results directly. Each brain was
annotated using desktop-based tools for labelling neuropil regions, locating landmarks in image
stacks and tracing fine neuronal processes both manually and automatically. The development
of the tracing and landmark annotation tools is described, and all of the tools used in this work
are available as free software. In order to compare and aggregate these data, which are from
many different brains, it is necessary to register each image stack onto some standard template
brain. Although this is a well-studied problem in medical imaging, these high resolution scans of
the central
fly brain are unusual in a number of respects. The relative effectiveness of various
methods currently available were tested on this data set. The best registrations were produced
by a method that generates free-form deformations based on B-splines (the Computational
Morphometry Toolkit), but for much faster registrations, the thin plate spline method based on
manual landmarks may be sufficient. The annotated and registered data allows us to produce
central complex template images and also files that accurately represent the possible central
complex connectivity apparent in these images. One interesting result to arise from these efforts
was evidence for a possible connection between the inferior region of the fan-shaped body and
the beta lobe of the mushroom body which had previously been missed in these GAL4 lines. In
addition, we can identify several connections which appear to be similar to those described in
[Hanesch et al., 1989], the canonical paper on the architecture of the Drosophila melanogaster
central complex, and describe for the first time their variation statistically. This registered data
was also used to suggest a method for classifying layers of expression within the fan-shaped body