The microsomal fraction of both dog and hog gastric mucosa
contains a K⁺ activated ATPase. ATP phosphorylates a peptide
of c̅ 100,000 Mᵣ in both species, and dephosphorylation
is stimulated by K⁺. By a combination of differential and
zonal density gradient centrifugation a membrane fraction
is produced containing almost exclusively this peptide
region. These vesicles, upon the addition of ATP, take
up H⁺ and extrude K⁺.
The action of ionophores such as
nigericin or valinomycin and the uptake of lipid permeable
anions such as thiocyanate or anilino-naphthosulfonic acid
indicate the lack of a potential difference during transport.
Reconstitution of this material into a planar bilayer
indicates that ATP activates a K⁺ conductance and
hence, in the presence of K⁺ also a low potential difference
is observed. These data suggest that this H⁺ pump
is non-electrogenic as prepared in the vesicular form.
Using an antibody obtained from rabbits immunized with
the highly purified membrane fractions, it was demonstrated
that this membrane was derived uniquely from gastric parietal
cells. Hence, based on the ability of this ATPase to
actively transport H⁺, its cellular origin and on the well
known K⁺ requirement for acid secretion in amphibia and
mammals, it is concluded that this ATPase is a component
of the HCl secretory mechanism of gastric mucosa.Paper I Secretion by in Vitro Amphibian Gastric Mucosa.
IN: Physiology of Gastric Secretion: NATO Institute,
Myren, J., ed, p. 186 -202, Oslo Press, 1968. •
Paper II Frog Gastric Mucosal ATPase. Proc. Soc. Exptl. Biol.
Med. 119: 1023 -1027, 1965. •
Paper III Action of SCN on Rat Liver Mitochondria. Proc. Soc.
Exptl. Biol. Med. 133: 456 -459, 1970. •
Paper IV Action of Thiocyanate on Gastric Mucosa in Vitro.
Biochim. Biophys. Acta 173: 509 -517, 1969. •
Paper V Role of ATP and ATPase in Gastric Acid Secretion.
IN: Gastric Secretion, (Sachs, G., Heinz, E.,
Ullrich, K.J., eds), Academic Press, New York,
pp. 321 -343, 1972. •
Paper VI Properties of ATPase of Gastric Mucosa. V. Preparation
of membranes and mitochondria by zonal centrifugation.
Biochim. Biophys. Acta 311: 545 -564, 1973. •
Paper VII Characterization of Gastric Mucosal Membranes.
VI. The presence of channel- forming substances.
Biochim. Biophys. Acta 332: 233 -247, 1974. •
Paper VIII Characterization of Gastric Mucosal Membranes.
Composition of gastric cell membranes and poly -
peptide fractionation using ionic and nonionic
detergents. Arch. Biochem. Biophys. 161: 456 -471,
1974. •
Paper IX Pronase Method for Isolation of Viable Cells From
Necturus Gastric Mucosa. Gastroenterology 61:
189 -200, 1971. •
Paper X Specific Effect of Acetylsalicylic Acid on Cation
Transport of Isolated Gastric Mucosal Cells.
Am. J. Physiol. 235: E16 -E21, 1978. •
Paper XI Studies on Adenyl Cyclase in Necturus Gastric Mucosa.
Arch. Biochem. Biophys. 143: 123 -126, 1971. •
Paper XII Adenyl and Guanyl Cyclase in Rabbit Gastric Mucosa.
Am. J. Physiol. 225: 1359 -1363, 1973. •
Paper XIII Action of Cholinergic Drugs on Necturus Gastric
Mucosa. Am. J. Physiol. 210: 1056 -1060, 1970. •
Paper XIV Action of Burimamide, a Histamine Antagonist, on
Acid Secretion in Vitro. Am. J. Physiol. 226: 898-
902, 1974. •
Paper XV Effects of Sodium Removal on Acid Secretion by the
Frog Gastric Mucosa. Proc. Soc. Exptl. Biol. Med.
123: 47 -52, 1966. •
Paper XVI Ion Transport by Amphibian Antrum in Vitro. I.
General Characteristics. Am. J. Physiol. 228:
1188 -1198, 1975. •
Paper XVII Quantitation of Conductance Pathways in Antral Gastric
Mucosa. J. Gen. Physiology 65: 645 -662, 1975.
Paper XVIII Properties of Gastric Antrum. III. Selectivity
and modification of shunt conductance. Gastroenterology
72: 72 -77, 1977. •
Paper XIX A Molecular Approach to Epithelial Conductance:
Gastric Mucosa. Aired Benzon Symp. V. Transport
Mechanisms in Epithelia, Munksgaard, Copenhagen,
pp. 257 -274, 1973. •
Paper XX Conductance Pathways in Epithelial Tissues.
Exp. Eye Res. 16: 241 -249, 1973. •
Paper XXI Electrical Properties of Isolated Cells of Necturus
Gastric Mucosa. Biochim. Biophys. Acta 241: 261 -272,
1971. •
Paper XXII Microelectrode Studies of Gastric Mucosa and Isolated
Gastric Cells. Symp. Med. Hoechst. IN:
Electrophysiology of Epithelial Cells, p. 257 -279,
1971. •
Paper XXIII Microelectrode Studies of Fundic Gastric Mucosa:
Cellular Coupling and Shunt Conductance. J. Membr.
Biol. 19: 105 -128, 1974, •
Paper XXIV The Action of Amytal on Frog Gastric Mucosa.
Biochim. Biophys. Acta 143: 522 -531, 1967.
Paper XXV Metabolism of Dog Gastric Mucosa. I. Nucleotide
Levels in Parietal Cells. J. Biol. Chem. 250:
8321 -8329, 1975. •
Paper XXVI Metabolism of Dog Gastric Mucosa. Levels of glycolytic
citric acid cycle and other intermediates. J. Biol.
Chem. 252: 8572 -8581, 1977. •
Paper XXVII Redox Involvement in Acid Secretion in the Amphibian
Gastric Mucosa. J. Membr. Biol. 35: 189 -204, 1977. •
Paper XXVIII REVIEW: H⁺ Transport by a Non-electrogenic Gastric
ATPase as a Model for Acid Secretion. Rev. Physiol.
Biochem. Pharmac. 79: 133 -167, 1977. •
Paper XXIX Characterization of Gastric Mucosal Membranes.
VIII. Localization of peptides by iodination and
phosphorylation. J. Biol. Chem. 250: 4802 -2809,
1975. •
Paper XXX Characterization of Gastric Mucosal Membranes.
IX. Fractionation and Purification of K⁺- ATPase
containing vesicles by zonal centrifugation and free
flow electrophoresis technique. Biochim. Biophys.
Acta. 465: 311 -330, 1977. •
Paper XXXI A Non -electrogenic H⁺ Pump in Plasma Membranes of
Hog Stomach. J. Biol. Chem. 251: 7690 -7698, 1976. •
Paper XXXII Proton Transport by Gastric Membrane Vesicles.
Biochim. Biophys. Acta 464: 313 -327, 1977. •
Paper XXXIII Cation Transport by Gastric H⁺ + K⁺ ATPase.
J. Membr. Biol. 32: 361 -381, 1977. •
Paper XXXIV Use of l-anilino-8-naphthalene sulfonate as a
Probe of Gastric Vesicle Transport. J. Membr.
Biol. 32: 301 -318, 1977. •
Paper XXXV Reconstitution of a Proton Pump from Gastric
Mucosa. J. Membr. Biol. 35: 285 -301, 1977. •
Paper XXXVI Metabolic and Membrane Aspects of Gastric H⁺
Transport. Gastroenterology 73: 931 -940, 1977. •
Paper XXXVII Tissue and Cell Localization of Hog Gastric Plasma
Membrane by Antibody Technique. Proc. Symp.
Gastric. Ion Transport, Special Suppl. Acta Physiol.
Scand (Obrink, K.J. and Flemstrom,-G., eds) p.
293 -305, 1978. •
Paper XXXVIII Transport Parameters of Gastric Vesicles.
Proc. Symp. Gastric Ion Transport, Special Suppl.
Acta Physiol. Scand. (Obrink, K.J. and Flemstrom,
G., eds) p. 409 -426, 1978. •
Paper XL Enzymic Modification of Gastric Transport ATPase.
IN: Frontiers of Biological Energetics (Dutton, P.L.,
Leigh, J., Scarpa, A., eds) Academic Press, New York,
Vol. 1, p. 545 -554, 1978. •
Paper XLI Transport Characteristics of Frog Gastric Membranes
Biochim. Biophys. Acta 551: 432 -447, 1979. •
Paper XLII Quantitation of Hydrogen Ion and Potential Gradients
in Gastric Plasma Membrane Vesicles. Biochemistry
17: 3345 -3353, 1978
