Pseudomonas aeruginosa ATCC 9027 was isolated in 1943 from a case of otitis externa and is commonly employed as a quality control strain for sterility, assessment of antibiofilm agents, and in vitro study of wound infection. Here, we present the 6.34-Mb draft genome sequence and highlight some pertinent genes that are associated with virulence

Jayal, Ambikesh

Johns, Benjamin E.

Maddocks, Sarah E.

Purdy, Kevin J.

Warwick Research Archives Portal Repository

     warwick.ac.uk/lib-publications      Original citation: Jayal, Ambikesh, Johns, Benjamin E., Purdy, Kevin J. and Maddocks, Sarah E.. (2017) Draft genome sequence of Pseudomonas aeruginosa ATCC 9027, originally isolated from an outer ear infection. Genome Announcements, 5 (48). e01397-17.  Permanent WRAP URL: http://wrap.warwick.ac.uk/96373                            Copyright and reuse: The Warwick Research Archive Portal (WRAP) makes this work of researchers of the University of Warwick available open access under the following conditions.  This article is made available under the Creative Commons Attribution 4.0 International license (CC BY 4.0) and may be reused according to the conditions of the license.  For more details see: http://creativecommons.org/licenses/by/4.0/    A note on versions: The version presented in WRAP is the published version, or, version of record, and may be cited as it appears here.  For more information, please contact the WRAP Team at: wrap@warwick.ac.uk  Draft Genome Sequence of Pseudomonasaeruginosa ATCC 9027, OriginallyIsolated from an Outer Ear InfectionAmbikesh Jayal,a Benjamin E. Johns,b Kevin J. Purdy,c Sarah E. MaddocksbDepartment of Computing and Information Systems, School of Management, Cardiff Metropolitan University,Cardiff, United Kingdoma; Department of Biomedical Sciences, Cardiff School of Sport and Health Sciences,Cardiff Metropolitan University, Cardiff, United Kingdomb; School of Life Sciences, University of Warwick,Coventry, United KingdomcABSTRACT Pseudomonas aeruginosa ATCC 9027 was isolated in 1943 from a case ofotitis externa and is commonly employed as a quality control strain for sterility, as-sessment of antibioﬁlm agents, and in vitro study of wound infection. Here, we pres-ent the 6.34-Mb draft genome sequence and highlight some pertinent genes thatare associated with virulence.Pseudomonas aeruginosa ATCC 9027 was originally isolated from a case of otitisexterna and is used as a quality control organism (https://www.lgcstandards-atcc.org/Products/Cells_and_Microorganisms/Bacteria.aspx). Despite its origin as a skinpathogen, this strain has been regarded as avirulent and has been utilized to producerhamnolipid surfactants (1, 2). Nonetheless, P. aeruginosa ATCC 9027 has been usedextensively to study wound infection in vitro, because it is an excellent bioﬁlm former(3). P. aeruginosa ATCC 9027 is closely related to PA7, a taxonomic outlier which exhibitsnumerous multidrug resistance mechanisms (4). PA7 lacks several factors associatedwith virulence, including a 36-gene cluster encoding the type III secretory system (5).Genomic DNA derived from a bioﬁlm of P. aeruginosa ATCC 9027 was puriﬁed usingthe GenElute bacterial genomic DNA extraction kit (Sigma-Aldrich). The sample librarywas prepared using an Illumina Nextera XT before DNA sequencing using an IlluminaMiSeq system. The genomic data were transferred to and stored in Illumina BaseSpace.The raw reads were processed for quality using Trimmomatic version 0.36 (6) andquality reports generated using FastQC (7). A 6,340,907-bp assembly with 126-foldcoverage was constructed using SPAdes version 3.8 (8) and run remotely using CloudInfrastructure for Microbial Bioinformatics (CLIMB) (9, 10). Quality assessment of assem-bly was done using QUAST (11). The assembly consists of 53 contigs (500 bp or more)with a mean GC content of 66.65%. The N50 and N75 values of the assembly were290,774 bp and 197,962 bp, respectively. The L50 and L75 values of the assembly were8 and 15, respectively. The annotation was performed with the NCBI ProkaryoticGenome Annotation Pipeline (http://www.ncbi.nlm.nih.gov/genome/annotation_prok),which predicted a total of 5,841 protein-coding genes and 58 tRNAs.The draft genome contained several virulence-associated genes, including ﬂg, pil,and gld (ﬂagellar and ﬁmbrial biosynthesis and motility [12, 13]), mviM (putativevirulence factor [http://www.uniprot.org/uniprot/Q1RD89]), hudR (virulence-associatedtranscriptional regulator [14]), the pvd operon (pyoverdin synthesis [15]), bvgS (viru-lence sensor protein [16]), mvfR-pqsR (multiple virulence factor regulator [17]), vreR(sigma factor regulator associated with virulence [18]), and hemO (heme oxygenase[19]), to name a few. The presence of these genes demonstrates that P. aeruginosaATCC 9027 should be classiﬁed as pathogenic, rather than avirulent, and could haveimplications for its use as a workhorse to produce surfactants.Received 6 November 2017 Accepted 6November 2017 Published 30 November2017Citation Jayal A, Johns BE, Purdy KJ, MaddocksSE. 2017. Draft genome sequence ofPseudomonas aeruginosa ATCC 9027, originallyisolated from an outer ear infection. GenomeAnnounc 5:e01397-17. https://doi.org/10.1128/genomeA.01397-17.Copyright © 2017 Jayal et al. This is an open-access article distributed under the terms ofthe Creative Commons Attribution 4.0International license.Address correspondence to Ambikesh Jayal,ajayal@cardiffmet.ac.uk, or Sarah E. Maddocks,smaddocks@cardiffmet.ac.uk.PROKARYOTEScrossmVolume 5 Issue 48 e01397-17 genomea.asm.org 1 on December 15, 2017 by guesthttp://genomea.asm.org/Downloaded from Accession number(s). This whole-genome shotgun project has been deposited atDDBJ/ENA/GenBank under the accession number PDLX00000000. The version de-scribed in this paper is version PDLX01000000. Unassembled reads are also availablefrom the NCBI Sequence Read Archive (SRA) under the accession number SRP120030.ACKNOWLEDGMENTSThis study was funded by a Cardiff Metropolitan University Research InnovationAward to S.E.M. This study has also used many resources from the Cloud Infrastructurefor Microbial Bioinformatics (CLIMB) project, which is funded by UK’s Medical ResearchCouncil and provides free cloud-based computing resources for analyzing microbialgenomics data.We gratefully acknowledge Lesley Ward and University of Warwick for performingIllumina MiSeq sequencing. We gratefully acknowledge the support provided by theCLIMB project and would like to thank the team managing and funding the CLIMBproject.REFERENCES1. Setoodeh P, Jahanmiri A, Eslamloueyan R, Niazi A, Ayatollahi SS, Aram F,Mahmoodi M, Hortamani A. 2014. Statistical screening of medium com-ponents for recombinant production of Pseudomonas aeruginosa ATCC9027 rhamnolipids by non-pathogenic cell factory Pseudomonas putidaKT2440. Mol Biotechnol 56:175–191. https://doi.org/10.1007/s12033-013-9693-1.2. Grosso-Becerra MV, González-Valdez A, Granados-Martínez MJ, MoralesE, Servín-González L, Méndez JL, Delgado G, Morales-Espinosa R, Ponce-Soto GY, Cocotl-Yañez M, Soberón-Chávez G. 2016. Pseudomonas aerugi-nosa ATCC 9027 in a non-virulent strain suitable for mono-rhamnolipidsproduction. Appl Microbiol Biotechnol 100:9995–10004. https://doi.org/10.1007/s00253-016-7789-9.3. Camplin AL, Maddocks SE. 2014. Manuka honey treatment of bioﬁlms ofPseudomonas aeruginosa results in the emergence of isolates with in-creased honey resistance. Ann Clin Microbiol Antimicrob 13:19. https://doi.org/10.1186/1476-0711-13-19.4. Mai-Prochnow A, Bradbury M, Murphy AB. 2015. Draft genome sequenceof Pseudomonas aeruginosa ATCC 9027 (DSM 1128), an important rham-nolipid surfactant producer and sterility testing strain. Genome Announc3:e01259-15. https://doi.org/10.1128/genomeA.01259-15.5. Roy PH, Tetu SG, Larouche A, Elbourne L, Tremblay S, Ren Q, Dodson R,Harkins D, Shay R, Watkins K, Mahamoud Y, Paulsen IT. 2010. Completegenome sequence of the multiresistant taxonomic outlier Pseudomonasaeruginosa PA7. PLoS One 5:e8842. https://doi.org/10.1371/journal.pone.0008842.6. Bolger AM, Lohse M, Usadel B. 2014. Trimmomatic: a ﬂexible trimmer forIllumina sequence data. Bioinformatics 30:2114–2120. https://doi.org/10.1093/bioinformatics/btu170.7. Andrews S. 2010. FastQC: a quality control tool for high throughputsequence data. http://www.bioinformatics.babraham.ac.uk/projects/fastqc.8. Nurk S, Bankevich A, Antipov D, Gurevich A, Korobeynikov A, Lapidus A,Prjibelsky A, Pyshkin A, Sirotkin A, Sirotkin Y, Stepanauskas R, McLean J,Lasken R, Clingenpeel SR, Woyke T, Tesler G, Alekseyev MA, Pevzner PA.2013. Assembling genomes and mini-metagenomes from highly chime-ric reads. In Deng M, Jiang R, Sun F, Zhang X (ed), Research in Compu-tational Molecular Biology (RECOMB) 2013. Lecture notes in computerscience, vol 7821. Springer, Berlin, Germany.9. Afgan E, Sloggett C, Goonasekera N, Makunin I, Benson D, Crowe M,Gladman S, Kowsar Y, Pheasant M, Horst R, Lonie A, Genomics VirtualLaboratory. 2015. A practical bioinformatics workbench for the cloud.PLoS One 10:e0140829. https://doi.org/10.1371/journal.pone.0140829.10. Connor TR, Loman NJ, Thompson S, Smith A, Southgate J, Poplawski R,Bull MJ, Richardson E, Ismail M, Thompson SE, Kitchen C, Guest M, BakkeM, Sheppard SK, Pallen MJ. 2016. CLIMB (the Cloud Infrastructure forMicrobial Bioinformatics): an online resource for the medical micro-biology community. Microb Genom 2:e000086. https://doi.org/10.1099/mgen.0.000086.11. Gurevich A, Saveliev V, Vyahhi N, Tesler G. 2013. QUAST: quality assess-ment tool for genome assemblies. Bioinformatics 29:1072–1075. https://doi.org/10.1093/bioinformatics/btt086.12. Drake D, Montie TC. 1988. Flagella, motility and invasive virulence ofPseudomonas aeruginosa. J Gen Microbiol 134:43–52. https://doi.org/10.1099/00221287-134-1-43.13. Persat A, Inclan YF, Engel JN, Stone HA, Gitai Z. 2015. Type IV pilimechanochemically regulate virulence factors in Pseudomonas aerugi-nosa. Proc Natl Acad Sci U S A 112:7563–7568. https://doi.org/10.1073/pnas.1502025112.14. Kim SH, Park SY, Heo YJ, Cho YH. 2008. Drosophila melanogaster-basedscreening for multihost virulence factors of Pseudomonas aeruginosaPA14 and identiﬁcation of a virulence attenuating factor, HudA. InfectImmun 76:4152–4162. https://doi.org/10.1128/IAI.01637-07.15. Meyer JM, Neely A, Stintzi A, Georges C, Holder IA. 1996. Pyoverdin isessential for virulence of Pseudomonas aeruginosa. Infect Immun 64:518–523.16. Linz B, Ivanov YV, Preston A, Brinkac L, Parkhill J, Kim M, Harris SR,Goodﬁeld LL, Fry NK, Gorringe AR, Nicholson TL, Register KB, Losada L,Harvill ET. 2016. Acquisition and loss of virulence-associated factorsduring genome evolution and speciation in three clades of Bordetellaspecies. BMC Genomics 17:767. https://doi.org/10.1186/s12864-016-3112-5.17. Déziel E, Gopalan S, Tampakaki AP, Lépine F, Padﬁeld KE, Saucier M, XiaoG, Rahme LG. 2005. The contribution of MvfR to Pseudomonas aerugi-nosa pathogenesis and quorum sensing-regulated genes are modulatedwithout affecting lasRI, rhlRI or the production of N-acyl-homoserine-lactones. Mol Microbiol 55:998–1014. https://doi.org/10.1111/j.1365-2958.2004.04448.x.18. Quesada JM, Otero-Asman JR, Bastiaansen KC, Civantos C, Llamas MA.2016. The activity of the Pseudomonas virulence regulator VreI is mod-ulated by the anti- factor VreR and the transcription factor PhoB. FrontMicrobiol 7:1159. https://doi.org/10.3389/fmicb.2016.01159.19. Zhu W, Hunt DJ, Richardson AR, Stojiljkovic I. 2000. Use of heme com-pounds as iron sources by pathogenic neisseriae requires the product ofthe hemO gene. J Bacteriol 182:439–447. https://doi.org/10.1128/JB.182.2.439-447.2000.Jayal et al.Volume 5 Issue 48 e01397-17 genomea.asm.org 2 on December 15, 2017 by guesthttp://genomea.asm.org/Downloaded from 

Draft genome sequence of Pseudomonas aeruginosa ATCC 9027, originally isolated from an outer ear infection

Pseudomonas aeruginosa ATCC 9027 was isolated in 1943 from a case of
otitis externa and is commonly employed as a quality control strain for sterility, assessment
of antibiofilm agents, and in vitro study of wound infection. Here, we present
the 6.34-Mb draft genome sequence and highlight some pertinent genes that
are associated with virulence

Johns, Benjamin

Maddocks, Sarah

Cardiff Metropolitan Research Repository (DSpace)

Draft Genome Sequence of Pseudomonasaeruginosa ATCC 9027, OriginallyIsolated from an Outer Ear InfectionAmbikesh Jayal,a Benjamin E. Johns,b Kevin J. Purdy,c Sarah E. MaddocksbDepartment of Computing and Information Systems, School of Management, Cardiff Metropolitan University,Cardiff, United Kingdoma; Department of Biomedical Sciences, Cardiff School of Sport and Health Sciences,Cardiff Metropolitan University, Cardiff, United Kingdomb; School of Life Sciences, University of Warwick,Coventry, United KingdomcABSTRACT Pseudomonas aeruginosa ATCC 9027 was isolated in 1943 from a case ofotitis externa and is commonly employed as a quality control strain for sterility, as-sessment of antibioﬁlm agents, and in vitro study of wound infection. Here, we pres-ent the 6.34-Mb draft genome sequence and highlight some pertinent genes thatare associated with virulence.Pseudomonas aeruginosa ATCC 9027 was originally isolated from a case of otitisexterna and is used as a quality control organism (https://www.lgcstandards-atcc.org/Products/Cells_and_Microorganisms/Bacteria.aspx). Despite its origin as a skinpathogen, this strain has been regarded as avirulent and has been utilized to producerhamnolipid surfactants (1, 2). Nonetheless, P. aeruginosa ATCC 9027 has been usedextensively to study wound infection in vitro, because it is an excellent bioﬁlm former(3). P. aeruginosa ATCC 9027 is closely related to PA7, a taxonomic outlier which exhibitsnumerous multidrug resistance mechanisms (4). PA7 lacks several factors associatedwith virulence, including a 36-gene cluster encoding the type III secretory system (5).Genomic DNA derived from a bioﬁlm of P. aeruginosa ATCC 9027 was puriﬁed usingthe GenElute bacterial genomic DNA extraction kit (Sigma-Aldrich). The sample librarywas prepared using an Illumina Nextera XT before DNA sequencing using an IlluminaMiSeq system. The genomic data were transferred to and stored in Illumina BaseSpace.The raw reads were processed for quality using Trimmomatic version 0.36 (6) andquality reports generated using FastQC (7). A 6,340,907-bp assembly with 126-foldcoverage was constructed using SPAdes version 3.8 (8) and run remotely using CloudInfrastructure for Microbial Bioinformatics (CLIMB) (9, 10). Quality assessment of assem-bly was done using QUAST (11). The assembly consists of 53 contigs (500 bp or more)with a mean GC content of 66.65%. The N50 and N75 values of the assembly were290,774 bp and 197,962 bp, respectively. The L50 and L75 values of the assembly were8 and 15, respectively. The annotation was performed with the NCBI ProkaryoticGenome Annotation Pipeline (http://www.ncbi.nlm.nih.gov/genome/annotation_prok),which predicted a total of 5,841 protein-coding genes and 58 tRNAs.The draft genome contained several virulence-associated genes, including ﬂg, pil,and gld (ﬂagellar and ﬁmbrial biosynthesis and motility [12, 13]), mviM (putativevirulence factor [http://www.uniprot.org/uniprot/Q1RD89]), hudR (virulence-associatedtranscriptional regulator [14]), the pvd operon (pyoverdin synthesis [15]), bvgS (viru-lence sensor protein [16]), mvfR-pqsR (multiple virulence factor regulator [17]), vreR(sigma factor regulator associated with virulence [18]), and hemO (heme oxygenase[19]), to name a few. The presence of these genes demonstrates that P. aeruginosaATCC 9027 should be classiﬁed as pathogenic, rather than avirulent, and could haveimplications for its use as a workhorse to produce surfactants.Received 6 November 2017 Accepted 6November 2017 Published 30 November2017Citation Jayal A, Johns BE, Purdy KJ, MaddocksSE. 2017. Draft genome sequence ofPseudomonas aeruginosa ATCC 9027, originallyisolated from an outer ear infection. GenomeAnnounc 5:e01397-17. https://doi.org/10.1128/genomeA.01397-17.Copyright © 2017 Jayal et al. This is an open-access article distributed under the terms ofthe Creative Commons Attribution 4.0International license.Address correspondence to Ambikesh Jayal,ajayal@cardiffmet.ac.uk, or Sarah E. Maddocks,smaddocks@cardiffmet.ac.uk.PROKARYOTEScrossmVolume 5 Issue 48 e01397-17 genomea.asm.org 1 on December 1, 2017 by guesthttp://genomea.asm.org/Downloaded from Accession number(s). This whole-genome shotgun project has been deposited atDDBJ/ENA/GenBank under the accession number PDLX00000000. The version de-scribed in this paper is version PDLX01000000. Unassembled reads are also availablefrom the NCBI Sequence Read Archive (SRA) under the accession number SRP120030.ACKNOWLEDGMENTSThis study was funded by a Cardiff Metropolitan University Research InnovationAward to S.E.M. This study has also used many resources from the Cloud Infrastructurefor Microbial Bioinformatics (CLIMB) project, which is funded by UK’s Medical ResearchCouncil and provides free cloud-based computing resources for analyzing microbialgenomics data.We gratefully acknowledge Lesley Ward and University of Warwick for performingIllumina MiSeq sequencing. We gratefully acknowledge the support provided by theCLIMB project and would like to thank the team managing and funding the CLIMBproject.REFERENCES1. Setoodeh P, Jahanmiri A, Eslamloueyan R, Niazi A, Ayatollahi SS, Aram F,Mahmoodi M, Hortamani A. 2014. Statistical screening of medium com-ponents for recombinant production of Pseudomonas aeruginosa ATCC9027 rhamnolipids by non-pathogenic cell factory Pseudomonas putidaKT2440. Mol Biotechnol 56:175–191. https://doi.org/10.1007/s12033-013-9693-1.2. Grosso-Becerra MV, González-Valdez A, Granados-Martínez MJ, MoralesE, Servín-González L, Méndez JL, Delgado G, Morales-Espinosa R, Ponce-Soto GY, Cocotl-Yañez M, Soberón-Chávez G. 2016. Pseudomonas aerugi-nosa ATCC 9027 in a non-virulent strain suitable for mono-rhamnolipidsproduction. Appl Microbiol Biotechnol 100:9995–10004. https://doi.org/10.1007/s00253-016-7789-9.3. Camplin AL, Maddocks SE. 2014. Manuka honey treatment of bioﬁlms ofPseudomonas aeruginosa results in the emergence of isolates with in-creased honey resistance. Ann Clin Microbiol Antimicrob 13:19. https://doi.org/10.1186/1476-0711-13-19.4. Mai-Prochnow A, Bradbury M, Murphy AB. 2015. Draft genome sequenceof Pseudomonas aeruginosa ATCC 9027 (DSM 1128), an important rham-nolipid surfactant producer and sterility testing strain. Genome Announc3:e01259-15. https://doi.org/10.1128/genomeA.01259-15.5. Roy PH, Tetu SG, Larouche A, Elbourne L, Tremblay S, Ren Q, Dodson R,Harkins D, Shay R, Watkins K, Mahamoud Y, Paulsen IT. 2010. Completegenome sequence of the multiresistant taxonomic outlier Pseudomonasaeruginosa PA7. PLoS One 5:e8842. https://doi.org/10.1371/journal.pone.0008842.6. Bolger AM, Lohse M, Usadel B. 2014. Trimmomatic: a ﬂexible trimmer forIllumina sequence data. Bioinformatics 30:2114–2120. https://doi.org/10.1093/bioinformatics/btu170.7. Andrews S. 2010. FastQC: a quality control tool for high throughputsequence data. http://www.bioinformatics.babraham.ac.uk/projects/fastqc.8. Nurk S, Bankevich A, Antipov D, Gurevich A, Korobeynikov A, Lapidus A,Prjibelsky A, Pyshkin A, Sirotkin A, Sirotkin Y, Stepanauskas R, McLean J,Lasken R, Clingenpeel SR, Woyke T, Tesler G, Alekseyev MA, Pevzner PA.2013. Assembling genomes and mini-metagenomes from highly chime-ric reads. In Deng M, Jiang R, Sun F, Zhang X (ed), Research in Compu-tational Molecular Biology (RECOMB) 2013. Lecture notes in computerscience, vol 7821. Springer, Berlin, Germany.9. Afgan E, Sloggett C, Goonasekera N, Makunin I, Benson D, Crowe M,Gladman S, Kowsar Y, Pheasant M, Horst R, Lonie A, Genomics VirtualLaboratory. 2015. A practical bioinformatics workbench for the cloud.PLoS One 10:e0140829. https://doi.org/10.1371/journal.pone.0140829.10. Connor TR, Loman NJ, Thompson S, Smith A, Southgate J, Poplawski R,Bull MJ, Richardson E, Ismail M, Thompson SE, Kitchen C, Guest M, BakkeM, Sheppard SK, Pallen MJ. 2016. CLIMB (the Cloud Infrastructure forMicrobial Bioinformatics): an online resource for the medical micro-biology community. Microb Genom 2:e000086. https://doi.org/10.1099/mgen.0.000086.11. Gurevich A, Saveliev V, Vyahhi N, Tesler G. 2013. QUAST: quality assess-ment tool for genome assemblies. Bioinformatics 29:1072–1075. https://doi.org/10.1093/bioinformatics/btt086.12. Drake D, Montie TC. 1988. Flagella, motility and invasive virulence ofPseudomonas aeruginosa. J Gen Microbiol 134:43–52. https://doi.org/10.1099/00221287-134-1-43.13. Persat A, Inclan YF, Engel JN, Stone HA, Gitai Z. 2015. Type IV pilimechanochemically regulate virulence factors in Pseudomonas aerugi-nosa. Proc Natl Acad Sci U S A 112:7563–7568. https://doi.org/10.1073/pnas.1502025112.14. Kim SH, Park SY, Heo YJ, Cho YH. 2008. Drosophila melanogaster-basedscreening for multihost virulence factors of Pseudomonas aeruginosaPA14 and identiﬁcation of a virulence attenuating factor, HudA. InfectImmun 76:4152–4162. https://doi.org/10.1128/IAI.01637-07.15. Meyer JM, Neely A, Stintzi A, Georges C, Holder IA. 1996. Pyoverdin isessential for virulence of Pseudomonas aeruginosa. Infect Immun 64:518–523.16. Linz B, Ivanov YV, Preston A, Brinkac L, Parkhill J, Kim M, Harris SR,Goodﬁeld LL, Fry NK, Gorringe AR, Nicholson TL, Register KB, Losada L,Harvill ET. 2016. Acquisition and loss of virulence-associated factorsduring genome evolution and speciation in three clades of Bordetellaspecies. BMC Genomics 17:767. https://doi.org/10.1186/s12864-016-3112-5.17. Déziel E, Gopalan S, Tampakaki AP, Lépine F, Padﬁeld KE, Saucier M, XiaoG, Rahme LG. 2005. The contribution of MvfR to Pseudomonas aerugi-nosa pathogenesis and quorum sensing-regulated genes are modulatedwithout affecting lasRI, rhlRI or the production of N-acyl-homoserine-lactones. Mol Microbiol 55:998–1014. https://doi.org/10.1111/j.1365-2958.2004.04448.x.18. Quesada JM, Otero-Asman JR, Bastiaansen KC, Civantos C, Llamas MA.2016. The activity of the Pseudomonas virulence regulator VreI is mod-ulated by the anti- factor VreR and the transcription factor PhoB. FrontMicrobiol 7:1159. https://doi.org/10.3389/fmicb.2016.01159.19. Zhu W, Hunt DJ, Richardson AR, Stojiljkovic I. 2000. Use of heme com-pounds as iron sources by pathogenic neisseriae requires the product ofthe hemO gene. J Bacteriol 182:439–447. https://doi.org/10.1128/JB.182.2.439-447.2000.Jayal et al.Volume 5 Issue 48 e01397-17 genomea.asm.org 2 on December 1, 2017 by guesthttp://genomea.asm.org/Downloaded from 

Draft Genome Sequence of Pseudomonas aeruginosa ATCC 9027, Originally Isolated from an Outer Ear Infection

ABSTRACT
          
            Pseudomonas aeruginosa
            ATCC 9027 was isolated in 1943 from a case of otitis externa and is commonly employed as a quality control strain for sterility, assessment of antibiofilm agents, and
            in vitro
            study of wound infection. Here, we present the 6.34-Mb draft genome sequence and highlight some pertinent genes that are associated with virulence.
          </jats:p

Ambikesh Jayal

Benjamin E. Johns

Kevin J. Purdy

Sarah E. Maddocks

Crossref

Genome Announcements

http://wrap.warwick.ac.uk/96373/7/WRAP-draft-genome-sequence-Pseudomonas-aeruginosa-ATCC-9027-Purdy-2017.pdf

Draft genome sequence of Pseudomonas aeruginosa ATCC 9027, originally isolated from an outer ear infection

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