The isolation of V-factor (NAD)-independent, serovar C-1 of Avibacterium paragallinarum from growing layer hens with infec- tious coryza, is reported for the first time. Av. paragallinarum serovar C-1 isolates were PCR confirmed and shared a unique genetic profile. Isolation of Av. paragallinarum NAD-independent biovar, serotyping and genotyping of isolates at the avian diagnostic laboratories are discussed