The function of latrines is still debated in many mammals, and in most cases latrine use is likely multi-functional. Functional hypotheses can be broadly divided into five categories that are not all mutually exclusive: resource defence, information centre/advertisement, landmarks/orientation, parasite control, or predator–prey interactions. To standardise all investigations into latrine function across species, we present a hypothesis-driven research framework based on the following five key categories of data that can, and we argue should, be collected from latrines: (1) spatial distribution patterns; (2) temporal usage patterns; (3) individual visitation and contribution patterns (to individual latrines); (4) behaviour of visitors; and (5) scent signal content and longevity. We suggest that our proposed approach offers a much-needed standardised structure to guide investigation into the function of latrine use in mammals