Biagini, D.

Biasato, I.

Biasibetti, E.

Capra, P.

Capucchio, M. T.

Dellarole, Stefania

Guarda, F.

Leporati, M.

Miniscalco, B.

Mioletti, S.

Tarantola, M.

Vincenti, M.

English

Institutional Research Information System University of Turin

Materials and Methods: Infection with EHV-5 was confirmed by PCR assays, while EHV-5 replication in tissues was demonstrated by using avirus-specific in situ hybridization (ISH) assay. Key inflammatory and immune cells, and activation of myofibroblasts and type IIpneumocytes were characterized by immunohistochemical staining, while collagen deposition was determined by Masson’s trichromehistochemical staining.Results: When compared with healthy controls, horses with EMPF had prominent myofibroblast activation and type II pneumocytehyperplasia together with a predominantly CD3+ T lymphocyte and monocyte/macrophage (Iba-1, pan-macrophage/dendritic cell; MAC387,recently infiltrating M1-like pro-inflammatory monocyte/macrophage) cellular infiltrate. When compared with EIPF, horses with EMPF had atwo to three folds increase in MAC387 and EHV-5 replicating exclusively within MAC387- pulmonary alveolar macrophages (PAM),presumably M2-like anti-inflammatory macrophages.Conclusions: These findings delineate EHV-5 tropism for long-lived, self-renewal PAM accompanied with a T lymphocyte-driven histiocyticresponse in horses with EMPF that will assist in the development of improved protocols for the diagnosis and control of this disease.Oral C - 3 :  INTRAMURAL CORONARY ARTERIOSCLEROSIS IN BEEF CATTLEREARED UNDER DIFFERENT HOUSING CONDITIONSI. Biasato , E. Biasibetti , Dellarole S. , Miniscalco B. , Mioletti S. , Tarantola M. , Biagini D. , Capra P. , Leporati M. , Vincenti M. , F.Guarda  and M. T. CapucchioDepartment of Veterinary Sciences, University of Turin, Italy, Department of Agricultural, Forestry and Food Sciences, University of Turin,Italy,  Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d’Aosta, Italy, Centro Regionale Antidoping e di Tossicologia “A.Bertinaria”, Italy, and Department of Chemistry, University of Turin, ItalyIntroduction:Intramural coronary arteriosclerosis has been reported in several species; no systematic studies are available in bovine.Rearing factors could influence the disease development. This study described coronary arteriosclerosis in regularly slaughtered beef cattlereared in different conditions.Materials and Methods: A total of 15 Piedmontese breed bulls reared in a tied stall housing system (G1), 15 Piedmontese (G2), 15 Blonded’Aquitaine (from France) (G3) and 15 cross-breed (from Ireland) (G4) reared in pen under different housing conditions and managementpractice were considered.  Blood parameters and dROMs were determined 3 weeks after the arrival of the animals and after 5 months. Hair20β-diidrocortisol was evaluated at the slaughter. Samples of interventricular septum, papillary muscles, ventricular free walls and atriawere submitted to histological evaluation (Haematoxylin & Eosin, Weigert VanGieson and AlcianPAS stains). Arteriosclerotic changes wereclassified using a semi-quantitative scoring system. GraphPad Prism® software was used to perform statistical analysis (P < 0.05).Results: Hematochemical parameters, dROMs and 20β-dihydrocortisol revealed that G2 and G3 groups were less stressed than G1 and G4.Likewise, interventricular septum and papillary muscles were significantly more affected (P< 0.001). G4 showed more severearteriosclerotic changes than others (P<0.001). A statistically significant association between G1 and G4 and the total number of affectedvessels was observed (P < 0.05). Independently of the group, Anitschkow cells were detected in the 83% of the pathological vessels. Conclusions:  Based on these findings, a potential relationship between the development of intramural coronary arteriosclerosis andhousing conditions-induced stress cannot be excluded.Oral C - 4 :  NEW INSIGHTS INTO THE PATHOGENESIS OF VASCULITIS INmalignant catarrhal feverH. Saura , M. Al-Saadi  J.P. Stewart  and A. KiparInstitute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich and Department of Infection Biology, Institute of Infection andGlobal Health, University of Liverpool, United KingdomIntroduction: Lymphoproliferative vasculitis has been described as the main histological feature of malignant catarrhal fever (MCF), withvirus infected T lymphocytes as the dominant cells. Deregulation of uninfected cells through infected lymphocytes, and autoreactiveimmune responses have been speculated as the underlying pathomechanisms.* * * * * * † ‡ # #@* ** †‡  #@*  †,  †  *†* †

Intramural coronary arteriosclerosis in beef cattle reared under different housing conditions.

https://iris.unito.it/bitstream/2318/1670517/1/Biasato%20et%20al.%20-%20ESVP%202017.pdf

Intramural coronary arteriosclerosis in beef cattle reared under different housing conditions.

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