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Medium-throughput production of recombinant human proteins: Protein production in insect cells
Authors
NA Burgess-Brown
O Gileadi
P Mahajan
C Strain-Damerell
Publication date
1 January 2014
Publisher
'Springer Science and Business Media LLC'
Doi
Abstract
This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA-DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell culture and describe the procedures involved in producing protein from liter-scale cultures. Although not discussed in this chapter, the same process can also be applied to integral membrane proteins (IMPs) with slight adaptations to the purification procedure. © 2014 Springer Science+Business Media, LLC
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Last time updated on 18/04/2020