Directed energy deposition (DED) is an emerging technology with repair applications in critical aerospace components. Mechanical properties of DED components have been shown to vary significantly through a part, making it difficult to achieve the level of process control required for these applications. Using thermal data captured in-situ, cooling rates and melt pool dimensions were calculated and related to the final grain structure, captured by EBSD. The changes in cooling rate explain the microstructural variation between different processing parameters and through the build height. A new approach, using a cumulative anisotropy factor was implemented and correlates the variation in hardness with grain structure. Two regimes were found depending on the linear heat input in 316L, with high linear heat input resulting in great amounts of mechanical anisotropy on the component level. The relationships between thermal signature and mechanical properties suggest close control of anisotropy could be achieved by monitoring and controlling the melt pool size using a coaxial camera